Department of Botany, University of Leicester, University Road, LE1 7RH, Leicester, UK.
Plant Cell Rep. 1990 Nov;9(7):406-9. doi: 10.1007/BF00232410.
The frequency and fidelity of Ac transposition, and that of its non-autonomous derivative Ds, were investigated in flax callus. Flax (Linum usitatissimum var. Antares) hypocotyls were transformed with Agrobacterium Ti plasmid vectors containing the Ac or Ds element inserted within the untranslated leader sequence of a chimaeric neomycin phosphotransferase II gene. Kanamycin resistant tissues were produced as a result of excision of Ac in around 35% of the total number of Ac-containing transformants. In contrast, no excision was observed from transformants containing the Ds element. Whilst Ac appears to have excised completely from T-DNAs, little evidence was found to infer reintegration of the Ac element into the genome.
我们研究了亚麻愈伤组织中转座子 Ac 的转座频率和保真度及其非自主衍生物 Ds 的转座频率和保真度。将含有 Ac 或 Ds 元件插入嵌合新霉素磷酸转移酶 II 基因非翻译前导序列的农杆菌 Ti 质粒载体转化亚麻（Linum usitatissimum var. Antares）下胚轴。约 35%的含 Ac 转化体中发生了 Ac 的切除，从而产生了卡那霉素抗性组织。相比之下，含 Ds 元件的转化体则没有观察到切除。尽管 Ac 似乎已从 T-DNA 中完全切除，但几乎没有证据表明 Ac 元件重新整合到基因组中。
