Laboratorium voor Genetica, Rijksuniversiteit Gent, B-9000 Gent, Belgium.
EMBO J. 1983;2(12):2143-50. doi: 10.1002/j.1460-2075.1983.tb01715.x.
A Ti plasmid mutant was constructed in which all the on-cogenic functions of the T-DNA have been deleted and replaced by pBR322. This Ti plasmid, pGV3850, still mediates efficient transfer and stabilization of its truncated T-DNA into infected plant cells. Moreover, integration and expression of this minimal T-DNA in plant cells does not interfere with normal plant cell differentiation. A DNA fragment cloned in a pBR vector can be inserted in the pGV3850 T-region upon a single recombination event through the pBR322 region of pGV3850 producing a co-integrate useful for the transformation of plant cells. Based upon these properties, pGV3850 is proposed as an extremely versatile vector for the introduction of any DNA of interest into plant cells.
构建了一个 Ti 质粒突变体，其中 T-DNA 的所有致癌功能已被删除，并被 pBR322 取代。这种 Ti 质粒，pGV3850，仍然介导其截断的 T-DNA 有效转移和稳定到感染的植物细胞中。此外，该最小 T-DNA 在植物细胞中的整合和表达不会干扰正常的植物细胞分化。克隆在 pBR 载体中的 DNA 片段可以通过 pGV3850 的 pBR322 区域在单个重组事件中插入 pGV3850 的 T 区，产生一个用于植物细胞转化的共整合体。基于这些特性，pGV3850 被提议作为一种非常通用的载体，用于将任何感兴趣的 DNA 引入植物细胞。
