Department of Science, Oregon Institute of Technology, 97601, Klamath Falls, Oregon.
Microb Ecol. 1980 Sep;6(3):217-26. doi: 10.1007/BF02010387.
Estimating microbial standing crops and microbial production in natural habitats has been difficult for microbial ecologists. The present paper describes a simple spectrophotometric assay based on the hydrolysis of fluorescein diacetate which estimates well the standing crops of microbial cells on coniferous needles and twigs. A technique is also presented for correlating optical density readings with actual dry weights of microbial cells epiphytic on needles, and thus for standardizing the assay. The assay shows promise of broad applicability to other microbial habitats.
估算自然生境中微生物的现存量和微生物的生产力一直是微生物生态学家面临的难题。本文介绍了一种基于荧光素二乙酸酯水解的简单分光光度测定法，该方法能很好地估算松针和嫩枝上微生物细胞的现存量。本文还提出了一种将针状叶上微生物细胞的吸光度读数与实际干重相关联的技术，并对该测定法进行了标准化。该测定法有望广泛应用于其他微生物生境。
