Department of Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY.
Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY.
Biotechnol J. 2014 Mar;9(3):386-395. doi: 10.1002/biot.201300288. Epub 2013 Dec 19.
High throughput (HT) platforms serve as a cost-efficient and rapid screening method for evaluating the effect of cell-culture conditions and screening of chemicals. We report the development of a HT cell-based microarray platform to assess the effect of culture conditions on Chinese hamster ovary (CHO) cells. Specifically, growth, transgene expression and metabolism of a GS/methionine sulphoximine (MSX) CHO cell line, which produces a therapeutic monoclonal antibody, was examined using a microarray system in conjunction with a conventional shake flask platform in a non-proprietary medium. The microarray system consists of 60-nL spots of cells encapsulated in alginate and separated in groups via an 8-well chamber system attached to the chip. Results show the non-proprietary medium developed allows cell growth, production, and normal glycosylation of recombinant antibody and metabolism of the recombinant CHO cells in both the microarray and shake flask platforms. In addition, 10.3 mM glutamate addition to the defined base medium results in lactate metabolism shift in the recombinant GS/MSX CHO cells in the shake flask platform. Ultimately, the results demonstrate that the HT microarray platform has the potential to be utilized for evaluating the impact of media additives on cellular processes, such as cell growth, metabolism, and productivity.
高通量 (HT) 平台可作为一种经济高效且快速的筛选方法，用于评估细胞培养条件的影响和化学品的筛选。我们报告了一种 HT 基于细胞的微阵列平台的开发，用于评估培养条件对中国仓鼠卵巢 (CHO) 细胞的影响。具体来说，使用微阵列系统结合传统摇瓶平台在非专有培养基中检查了产生治疗性单克隆抗体的 GS/亚甲硫氨酸亚砜 (MSX) CHO 细胞系的生长、转基因表达和代谢。微阵列系统由封装在藻酸盐中的 60-nL 点细胞组成，并通过附接到芯片的 8 孔室系统分组分离。结果表明，开发的非专有培养基允许细胞生长、生产和重组抗体的正常糖基化以及重组 CHO 细胞在微阵列和摇瓶平台中的代谢。此外，在定义的基础培养基中添加 10.3mM 谷氨酸会导致摇瓶平台中重组 GS/MSX CHO 细胞的乳酸代谢转变。最终，结果表明 HT 微阵列平台有可能用于评估培养基添加剂对细胞过程的影响，例如细胞生长、代谢和生产力。
