Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.
Korean J Physiol Pharmacol. 2013 Oct;17(5):469-77. doi: 10.4196/kjpp.2013.17.5.469. Epub 2013 Oct 17.
This study investigated effect of extract containing quercetin-3-O-β-D-glucuronopyranoside from Rumex Aquaticus Herba (ECQ) against chronic gastritis in rats. To produce chronic gastritis, the animals received a daily intra-gastric administration of 0.1 ml of 0.15% iodoacetamide (IA) solution for 7 days. Daily exposure of the gastric mucosa to IA induced both gastric lesions and significant reductions of body weight and food and water intake. These reductions recovered with treatment with ECQ for 7 days. ECQ significantly inhibited the elevation of the malondialdehyde levels and myeloperoxidase activity, which were used as indices of lipid peroxidation and neutrophil infiltration. ECQ recovered the level of glutathione, activity of superoxide dismutase (SOD), and expression of SOD-2. The increased levels of total NO concentration and iNOS expression in the IA-induced chronic gastritis were significantly reduced by treatment with ECQ. These results suggest that the ECQ has a therapeutic effect on chronic gastritis in rats by inhibitory actions on neutrophil infiltration, lipid peroxidation and various steps of reactive oxygen species (ROS) generation.
本研究调查了从水飞蓟草（ECQ）中提取的含有槲皮素-3-O-β-D-葡萄糖醛酸苷的提取物对大鼠慢性胃炎的作用。为了产生慢性胃炎，动物每天接受 0.15%碘乙酰胺（IA）溶液 0.1ml 的胃内给药，持续 7 天。IA 每日暴露于胃黏膜会引起胃损伤以及体重、食物和水摄入的显著减少。用 ECQ 治疗 7 天可恢复这些减少。ECQ 可显著抑制丙二醛水平和髓过氧化物酶活性的升高，这两者被用作脂质过氧化和嗜中性粒细胞浸润的指标。ECQ 恢复了谷胱甘肽水平、超氧化物歧化酶（SOD）的活性和 SOD-2 的表达。在 IA 诱导的慢性胃炎中，总 NO 浓度和 iNOS 表达的升高水平通过用 ECQ 治疗而显著降低。这些结果表明，ECQ 通过抑制嗜中性粒细胞浸润、脂质过氧化和活性氧（ROS）生成的各个步骤对大鼠慢性胃炎具有治疗作用。
