肠道上皮共培养物的蛋白质表达谱：功能化碳纳米管暴露的影响。
Protein expression profiles of intestinal epithelial co-cultures: effect of functionalised carbon nanotube exposure.
作者信息
Lai Xianyin, Blazer-Yost Bonnie L, Clack James W, Fears Sharry L, Mitra Somenath, Ntim Susana Addo, Ringham Heather N, Witzmann Frank A
机构信息
Department of Cellular and Integrative Physiology, Indiana University School of Medicine, 1345 West 16th Street, Indianapolis IN 46202, USA,
出版信息
Int J Biomed Nanosci Nanotechnol. 2013;3(1-2). doi: 10.1504/IJBNN.2013.054508.
To assess the biological effects of low level, water dispersible, functionalised carbon nanotube (f-CNT) exposure in an in vitro model simulating the digestive tract, cellular protein expression was quantified and compared using label-free quantitative mass spectrometry (LFQMS). Co-cultured cells were exposed to well-characterised SWCNT-COOH, MWCNT-COOH, and MWCNT-PVP. The relative expression of 2,282 unique proteins was compared across the dose groups. 428 proteins were found to be differentially expressed. At the high dose, the extent of differential protein expression was CNT-specific and directly related to CNT colloidal stability. Cells responded to low level MWCNT-PVP exposure with three-fold greater differential expression. Bioinformatic analysis indicated significant and f-CNT-specific effects on relevant molecular and cellular functions and canonical pathways, with little overlap across f-CNT type and in the absence of overt toxicity.
为了在模拟消化道的体外模型中评估低水平、水分散性、功能化碳纳米管（f-CNT）暴露的生物学效应，使用无标记定量质谱（LFQMS）对细胞蛋白质表达进行定量和比较。将共培养的细胞暴露于特征明确的单壁碳纳米管-羧基（SWCNT-COOH）、多壁碳纳米管-羧基（MWCNT-COOH）和多壁碳纳米管-聚乙烯吡咯烷酮（MWCNT-PVP）。比较了各剂量组中2282种独特蛋白质的相对表达。发现428种蛋白质存在差异表达。在高剂量下，差异蛋白质表达的程度具有碳纳米管特异性，且与碳纳米管的胶体稳定性直接相关。细胞对低水平MWCNT-PVP暴露的反应是差异表达增加了三倍。生物信息学分析表明，对相关分子和细胞功能以及经典途径存在显著且f-CNT特异性的影响，不同f-CNT类型之间几乎没有重叠，且不存在明显毒性。
Zotero 插件