Department of Brain and Cognitive Sciences, College of Natural Sciences, Seoul National University, Seoul 151-746, South Korea.
Mol Pain. 2013 Nov 14;9:58. doi: 10.1186/1744-8069-9-58.
Voltage gated calcium channels (VGCCs) are well known for its importance in synaptic transmission in the peripheral and central nervous system. However, the role of different VGCCs in the anterior cingulate cortex (ACC) has not been studied. Here, we use a multi-electrode array recording system (MED64) to study the contribution of different types of calcium channels in glutamatergic excitatory synaptic transmission in the ACC. We found that only the N-type calcium channel blocker ω-conotoxin-GVIA (ω-Ctx-GVIA) produced a great inhibition of basal synaptic transmission, especially in the superficial layer. Other calcium channel blockers that act on L-, P/Q-, R-, and T-type had no effect. We also tested the effects of several neuromodulators with or without ω-Ctx-GVIA. We found that N-type VGCC contributed partially to (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid- and (R)-Baclofen-induced synaptic inhibition. By contrast, the inhibitory effects of 2-Chloroadenosine and carbamoylcholine chloride did not differ with or without ω-Ctx-GVIA, indicating that they may act through other mechanisms. Our results provide strong evidence that N-type VGCCs mediate fast synaptic transmission in the ACC.
电压门控钙通道（VGCCs）在周围和中枢神经系统的突触传递中具有重要作用。然而，不同类型的 VGCC 在扣带前皮质（ACC）中的作用尚未得到研究。在这里，我们使用多电极阵列记录系统（MED64）研究不同类型钙通道在 ACC 中谷氨酸能兴奋性突触传递中的作用。我们发现只有 N 型钙通道阻断剂 ω-芋螺毒素-GVIA（ω-Ctx-GVIA）对基础突触传递产生了很大的抑制作用，尤其是在浅层。作用于 L、P/Q、R 和 T 型的其他钙通道阻断剂没有效果。我们还测试了几种神经调质在有无 ω-Ctx-GVIA 时的作用。我们发现 N 型 VGCC 部分参与了（1S，3R）-1-氨基环戊烷-1,3-二羧酸和（R）-巴氯芬诱导的突触抑制。相比之下，2-氯腺苷和氨甲酰胆碱氯化物的抑制作用在有无 ω-Ctx-GVIA 时没有差异，表明它们可能通过其他机制发挥作用。我们的结果提供了强有力的证据，表明 N 型 VGCC 介导了 ACC 中的快速突触传递。
