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莱茵衣藻的硝酸还原酶系统。

The nitrate-reducing enzyme system of Chlamydomonas reinhardii.

作者信息

Barea J L, Cárdenas J

出版信息

Arch Microbiol. 1975 Sep 30;105(1):21-5. doi: 10.1007/BF00447107.

DOI:10.1007/BF00447107
PMID:242292
Abstract

In Chlamydomonas reinhardii the reduction of nitrate to ammonia occurs in two independent enzymatic steps: 1. the two-electrons reduction of nitrate to nitrite catalyzed by NADH-nitrate reductase, and, 2. the six-electrons reduction of nitrite to ammonia catalyzed by ferredoxin-nitrite reductase. Both enzymes have been purified and characterized, and some of their properties have been studied.

摘要

在莱茵衣藻中,硝酸盐还原为氨的过程分两个独立的酶促步骤进行:1. 由NADH-硝酸盐还原酶催化的硝酸盐双电子还原为亚硝酸盐;2. 由铁氧还蛋白-亚硝酸盐还原酶催化的亚硝酸盐六电子还原为氨。这两种酶均已被纯化并进行了表征,且对它们的一些特性进行了研究。

相似文献

1
The nitrate-reducing enzyme system of Chlamydomonas reinhardii.莱茵衣藻的硝酸还原酶系统。
Arch Microbiol. 1975 Sep 30;105(1):21-5. doi: 10.1007/BF00447107.
2
Purification and properties of the Neurospora crassa assimilatory nitrite reductase.粗糙脉孢菌同化型亚硝酸还原酶的纯化及性质
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3
Nitrate reductase from Spinacea oleracea. FAD and the inactivation by NAD (P) H.
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4
Reduced nicotinamide-adenine dinucleotide-nitrite reductase from Azotobacter chroococcum.来自褐球固氮菌的还原型烟酰胺腺嘌呤二核苷酸-亚硝酸盐还原酶。
Biochem J. 1973 Aug;133(4):701-8. doi: 10.1042/bj1330701.
5
The bacterial nitrate reductases. Solubilization, purification and properties of the enzyme A of Escherichia coli K 12.细菌硝酸还原酶。大肠杆菌K12中酶A的溶解、纯化及特性
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6
Ferredoxin-dependent photosynthetic reduction of nitrate and nitrite by particles of Anacystis nidulans.费氏铁氧还蛋白依赖的集胞藻颗粒对硝酸盐和亚硝酸盐的光合还原作用。
Mol Cell Biochem. 1976 Feb 25;10(3):161-9. doi: 10.1007/BF01731687.
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Regulation of the nitrate-reducing system enzymes in wild-type and mutant strains of Chlamydomonas reinhardii.莱茵衣藻野生型和突变株中硝酸盐还原系统酶的调控
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Biochem Biophys Res Commun. 1977 Sep 9;78(1):185-93. doi: 10.1016/0006-291x(77)91238-4.
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The assimilatory nitrate reductase from the phototrophic bacterium, Rhodobacter capsulatus E1F1, is a flavoprotein.来自光合细菌荚膜红细菌E1F1的同化型硝酸还原酶是一种黄素蛋白。
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A reduced pyridine nucleotides-diaphorase activity associated to the assimilatory nitrite reductase complex from Neurospora crassa.与粗糙脉孢菌同化亚硝酸还原酶复合物相关的吡啶核苷酸二磷酸酶活性降低。
Arch Microbiol. 1976 Sep 1;109(3):237-42. doi: 10.1007/BF00446634.

引用本文的文献

1
Genetic analysis of nitrate reductase-deficient mutants in Chlamydomonas reinhardii.莱茵衣藻硝酸盐还原酶缺陷突变体的遗传分析。
Curr Genet. 1984 Oct;8(8):635-40. doi: 10.1007/BF00395710.
2
Salinity tolerance of Picochlorum atomus and the use of salinity for contamination control by the freshwater cyanobacterium Pseudanabaena limnetica.微囊藻和淡水蓝藻 Pseudanabaena limnetica 耐盐性及其用于控制污染。
PLoS One. 2013 May 7;8(5):e63569. doi: 10.1371/journal.pone.0063569. Print 2013.
3
Kinetic Characterization of Nitrite Uptake and Reduction by Chlamydomonas reinhardtii.

本文引用的文献

1
[Iron content and electron donor specificity of the nitrate reductase from Ankistrodesmus].[绿藻的硝酸还原酶的铁含量及电子供体特异性]
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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The genetics and cytology of Chlamydomonas.衣藻的遗传学与细胞学
莱茵衣藻摄取和还原亚硝酸盐的动力学特征。
Plant Physiol. 1986 Dec;82(4):904-8. doi: 10.1104/pp.82.4.904.
4
In vitro reconstitution of electron transport from glucose-6-phosphate and NADPH to nitrite.体外重建从6-磷酸葡萄糖和NADPH到亚硝酸盐的电子传递。
Plant Physiol. 1998 May;117(1):303-9. doi: 10.1104/pp.117.1.303.
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In vivo complementation analysis of nitrate reductase-deficient mutants in Chlamydomonas reinhardtii.莱茵衣藻硝酸还原酶缺陷型突变体的体内互补分析
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6
Stable nuclear transformation of Chlamydomonas using the Chlamydomonas gene for nitrate reductase.利用衣藻硝酸还原酶基因对衣藻进行稳定的核转化。
J Cell Biol. 1989 Dec;109(6 Pt 1):2589-601. doi: 10.1083/jcb.109.6.2589.
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Ferredoxin-nitrite reductase from spinach.来自菠菜的铁氧化还原蛋白-亚硝酸盐还原酶。
Biochim Biophys Acta. 1966 Apr 12;118(1):58-71. doi: 10.1016/s0926-6593(66)80144-3.
5
Flavin nucleotide nitrate reductase from spinach.菠菜中的黄素核苷酸硝酸还原酶。
Biochim Biophys Acta. 1965 Sep 27;109(1):79-85. doi: 10.1016/0926-6585(65)90092-0.
6
[Preliminary observations concerning the nitrate reductase of Ankistrodesmus braunii].[关于布朗栅藻硝酸还原酶的初步观察]
Arch Mikrobiol. 1970;70(2):147-56. doi: 10.1007/BF00412205.
7
Preparation and characterization of a soluble nitrate reductase from Azotobacter chroococcum.来自褐球固氮菌的可溶性硝酸还原酶的制备与表征
Arch Mikrobiol. 1973 Jun 25;91(4):287-304. doi: 10.1007/BF00425049.
8
Regulation by ammonia of nitrate reductase synthesis and activity in Chlamydomonas reinhardi.莱茵衣藻中氨对硝酸还原酶合成和活性的调控
Biochem Biophys Res Commun. 1972 Aug 21;48(4):996-1003. doi: 10.1016/0006-291x(72)90707-3.
9
Purification and properties of nitrite reductase from spinach leaves.菠菜叶中亚硝酸还原酶的纯化及性质
FEBS Lett. 1972 Jun 15;23(2):131-5. doi: 10.1016/0014-5793(72)80322-3.
10
Mechanism of nitrate reduction in Chlorella.小球藻中硝酸盐还原的机制。
Biochem Biophys Res Commun. 1969 Sep 10;36(6):980-6. doi: 10.1016/0006-291x(69)90300-3.