Key Laboratory of Pharmaceutical Quality Control of Hebei Province & College of Pharmacy, Hebei University , Baoding, 071002, China.
J Agric Food Chem. 2013 Dec 11;61(49):11974-80. doi: 10.1021/jf403759t. Epub 2013 Nov 26.
New water-compatible molecularly imprinted microspheres were synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization using 3-(2-carboxyethylsulfanylthiocarbonyl-sulfanyl) propionic acid as a hydrophilic chain-transfer agent, and employed as the sorbent of pipet tip molecularly imprinted solid-phase extraction (PT-MISPE) for rapid extraction and screening of ofloxacin, pefloxacin, norfloxacin, ciprofloxacin, and enrofloxacin in eggs. In comparison to conventional SPE methods, the presented PT-MISPE showed special selectivity, easy operation, and accessible device without expensive SPE apparatus. The presented PT-MISPE method combined advantages of dummy molecularly imprinted polymers and pipet tip solid-phase extraction. The presented method was linear over a calibration range of 25-2500 μg/kg with the limits of detections of 0.53-1.07 μg/kg. Good recoveries (89.1-102.5%) were achieved with relative standard deviations of 2.6-4.8%.
新型水相兼容的分子印迹微球通过可逆加成-断裂链转移（RAFT）聚合反应，以 3-（2-羧乙基硫代磺酰基硫代）丙磺酸作为亲水性链转移剂进行合成，并作为管嘴型分子印迹固相萃取（PT-MISPE）的吸附剂，用于快速提取和筛选鸡蛋中的氧氟沙星、培氟沙星、诺氟沙星、环丙沙星和恩诺沙星。与传统 SPE 方法相比，所提出的 PT-MISPE 具有特殊的选择性、易于操作和可及的装置，而无需昂贵的 SPE 仪器。所提出的 PT-MISPE 方法结合了虚拟分子印迹聚合物和管嘴型固相萃取的优点。该方法在 25-2500μg/kg 的校准范围内呈线性，检测限为 0.53-1.07μg/kg。回收率良好（89.1-102.5%），相对标准偏差为 2.6-4.8%。
