慢性新斯的明治疗对大鼠骨骼肌神经肌肉接头处通道特性的影响。
The effect of chronic neostigmine treatment on channel properties at the rat skeletal neuromuscular junction.
作者信息
Gwilt M, Wray D
出版信息
Br J Pharmacol. 1986 May;88(1):25-31. doi: 10.1111/j.1476-5381.1986.tb09467.x.
We have studied the effects of chronic neostigmine treatment on single channel properties at the rat skeletal neuromuscular junction. Rats received 0.86 mg kg-1 neostigmine (s.c.) daily for 9-11 days. Microelectrode recordings were then made from the extensor digitorum longus muscle. The amplitude of miniature endplate potentials was significantly reduced in muscles from neostigmine-treated rats as compared with controls. Acetylcholine (2-5 microM) applied in the bath produced a depolarization and associated channel opening frequency (from voltage noise analysis) which were significantly reduced in neostigmine-treated muscles with respect to controls. The depolarization resulting from the opening of a single channel (from voltage noise analysis) and single channel open time and conductance (from current noise analysis) were not significantly changed by chronic neostigmine treatment. It is concluded that chronic neostigmine treatment causes an adaptive reduction in the number of functional acetylcholine receptors at the endplate without otherwise affecting single channel properties themselves.
我们研究了慢性新斯的明治疗对大鼠骨骼肌神经肌肉接头单通道特性的影响。大鼠每天皮下注射0.86 mg/kg新斯的明，持续9 - 11天。然后从趾长伸肌进行微电极记录。与对照组相比，新斯的明治疗大鼠的肌肉中微小终板电位的幅度显著降低。浴槽中加入乙酰胆碱（2 - 5 microM）会产生去极化以及相关的通道开放频率（通过电压噪声分析），相对于对照组，新斯的明治疗的肌肉中这些指标显著降低。由单通道开放引起的去极化（通过电压噪声分析）以及单通道开放时间和电导（通过电流噪声分析）并未因慢性新斯的明治疗而发生显著变化。得出的结论是，慢性新斯的明治疗会导致终板处功能性乙酰胆碱受体数量适应性减少，而不会以其他方式影响单通道特性本身。
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