United AgriSeeds, Inc., P.O. Box 4011, 61820, Champaign, IL, USA.
Theor Appl Genet. 1988 Jul;76(1):157-9. doi: 10.1007/BF00288847.
Anther culture of a three-way cross, (H99 × FR16) × Pa91, resulted in the regeneration of two anther-derived plants which were crossed to produce an F1 progeny. Fourteen S1 families derived from this cross were evaluated for their anther culturability. Dramatic increases in the level of androgenesis, expressed as the percentage of cultured anthers which produced embryo-like structures, were observed. An overall mean response frequency of 23.4% was observed for the S1 families. This was compared to a 3.5% response in the original three-way cross. These results demonstrate that genetic improvement of in vitro androgenesis in maize is possible and that anther culture per se constitutes a procedure for selecting genes which favor increased levels of response.
三交种（H99×FR16）×Pa91 的花药培养产生了两个花药再生植株，这两个植株被杂交以产生 F1 后代。对来自该杂交的 14 个 S1 家系进行了花药培养能力的评估。观察到，雄核发育的水平显著增加，表现为培养的花药产生胚状体结构的百分比增加。S1 家系的总体平均响应频率为 23.4%。与原始三交种的 3.5%的响应相比，这是一个显著的提高。这些结果表明，在玉米中体外雄核发育的遗传改良是可能的，并且花药培养本身就是一种选择有利于提高响应水平的基因的方法。
