利用同工酶数据评价关于雀舌黄杨（豆科）起源的假说。

Evaluation of hypotheses concerning the origin of Lotus corniculatus (Fabaceae) using isoenzyme data.

机构信息

Department of Plant Science, Macdonald College of McGill University, Ste. Anne de Bellevue, P. O. Box 4000, H9X 1C0, Quebec, Canada.

出版信息

Theor Appl Genet. 1988 Aug;76(2):267-76. doi: 10.1007/BF00257855.

DOI:10.1007/BF00257855

PMID:24232115

Abstract

An isoenzyme survey was conducted for several geographically dispersed accessions of four diploid Lotus species, L. alpinus Schleich., L. japonicus (Regel) Larsen, L. tenuis Waldst. et Kit and L. uliginosus Schkuhr, and for the tetraploid L. corniculatus L., in order to ascertain whether isoenzyme data could offer additional evidence concerning the origin of L. corniculatus. Seven enzyme systems were examined using horizontal starch gel electrophoresis. These were PGI, TPI, MDH, IDH, PGM, 6-PGDH, and ME. Lotus uliginosus had monomorphic unique alleles, that were not found within L. corniculatus, at 7 loci. These loci and alleles are: Tpi1-112, Pgm1,2-110, Pgm3-82, Mdh3-68, 6-Pgdh1-110, 6-Pgdh2-98,95, and Me2-100. Other diploid taxa contained alleles found in L. corniculatus for these and other loci. The implications of the isoenzyme data to theories on the origin of L. corniculatus are discussed.

摘要

进行了同工酶调查，涉及四个二倍体 Lotus 物种（高山 Lotus 种 Schleich.、日本 Lotus 种（Regel）Larsen、纤细 Lotus 种 Waldst. et Kit 和沼泽 Lotus 种 Schkuhr）的地理上分散的多个品系，以及四倍体 Lotus 种 corniculatus L.，以确定同工酶数据是否能提供有关 Lotus corniculatus 起源的额外证据。使用水平淀粉凝胶电泳检查了 7 种酶系统。这些是 PGI、TPI、MDH、IDH、PGM、6-PGDH 和 ME。沼泽 Lotus 种在 7 个基因座上具有独特的单态等位基因，这些等位基因在 Lotus corniculatus 中不存在。这些基因座和等位基因是：Tpi1-112、Pgm1,2-110、Pgm3-82、Mdh3-68、6-Pgdh1-110、6-Pgdh2-98、95 和 Me2-100。其他二倍体类群包含这些和其他基因座中存在于 Lotus corniculatus 中的等位基因。讨论了同工酶数据对 Lotus corniculatus 起源理论的影响。