Laboratory of Plant Breeding, Faculty of Agriculture, The University of Tokyo, Yayoi, Bunkyo-ku, 113, Tokyo, Japan.
Theor Appl Genet. 1988 Aug;76(2):293-8. doi: 10.1007/BF00257858.
Two techniques were useful in overcoming hybrid inviability between N. repanda and N. tabacum. These techniques combine gamma-ray irradiation to pollen or to egg cells (in ovules) with in vitro culture of fertilized ovules. When in vitro culture of fertilized ovules from in situ hybridization of N. repanda x N. tabacum was combined without gamma-ray irradiation to pollen or to egg cells (in ovules), all of the resulting seedlings developed chlorosis and died. Furthermore, in the case of in situ hybridization of N. repanda x N. tabacum with gamma-ray irradiated N. tabacum pollen, no viable seeds were obtained. By using both techniques, combining gamma-ray irradiation to N. tabacum pollen or to egg cells in (N. repanda ovules) with in vitro culture of fertilized ovules, we were successful in obtaining flowering hybrid plants. Thus, it appears that it may be possible to overcome hybrid inviability to a certain extent using both the pollen irradiation technique and the egg cell irradiation technique, i.e., gamma-ray irradiation to pollen or to egg cells (in ovules) before pollination and in vitro culture of fertilized ovules.
两种技术有助于克服 repanda 烟和 tabacum 烟之间的杂种不育性。这些技术将花粉或卵细胞（在胚珠中）的伽马射线照射与胚珠受精后的体外培养相结合。当将 repanda x tabacum 的原位杂交的受精胚珠进行体外培养而不进行花粉或卵细胞（在胚珠中）的伽马射线照射时，所有得到的幼苗都出现黄化并死亡。此外，在用伽马射线辐照的 tabacum 花粉进行 repanda x tabacum 的原位杂交的情况下，没有获得有活力的种子。通过同时使用这两种技术，将伽马射线照射到 tabacum 花粉或（repanda 胚珠中的）卵细胞中，并对受精胚珠进行体外培养，我们成功地获得了开花的杂种植物。因此，似乎可以通过花粉照射技术和卵细胞照射技术在一定程度上克服杂种不育性，即在授粉前对花粉或卵细胞（在胚珠中）进行伽马射线照射，并对受精胚珠进行体外培养。
