Department of Horticulture and Genetics Program, Oregon State University, Corvallis, Oregon, USA.
Theor Appl Genet. 1978 Sep;52(5):209-15. doi: 10.1007/BF00273891.
The influence of genotypic combinations on the growth of hybrid embryos between Phaseolus vulgaris and P. lunatus, and between P. vulgaris and P. acutifolius was examined. All embryos obtained from P. vulgaris × P. lunatus crosses developed only to a stage which appears to be comparable to the pre-heart-shape stage of selfed embryos. Reciprocal crosses were attempted, but pods abscised at a very early stage. Embryos derived from P. vulgaris × P. acutifolius and reciprocal crosses attained the cotyledon stage although no mature seeds were formed. A distinct characteristic of these embryos was the uneven development of the two cotyledons. The rate of growth and final size of these hybrid embryos seemed to be influenced by the genotypes of both parents.Immature embryos were cultured on defined medium and the effects of glutamine and gibberellin (GA3) were examined. Glutamine was effective in increasing the survival rate; gibberellin had no apparent effect. Plants derived from cultured embryos of P. vulgaris × P. lunatus, P. vulgaris × P. acutifolius and P. acutifolius × P. vulgaris were obtained.
考察了基因型组合对菜豆和绿豆杂种胚以及菜豆和兵豆杂种胚生长的影响。所有来自菜豆×绿豆杂交的胚仅发育到类似于自交胚心形前期的阶段。尝试了正反交，但豆荚在早期就脱落了。尽管没有形成成熟的种子，但来自菜豆×兵豆和正反交的胚达到了子叶阶段。这些胚的一个明显特征是两个子叶的不均匀发育。这些杂种胚的生长速度和最终大小似乎受到双亲基因型的影响。将未成熟的胚在定义的培养基上培养，并检查了谷氨酰胺和赤霉素（GA3）的作用。谷氨酰胺可有效提高存活率；赤霉素没有明显的效果。从菜豆×绿豆、菜豆×兵豆和兵豆×菜豆的培养胚中获得了植株。
