Department of Biophysics, József Attila University, H-6722, Szeged, Hungary.
Planta. 1986 Mar;169(1):123-9. doi: 10.1007/BF01369783.
The effects of the pyridazinone compound SAN 9785 on the photosynthetic competence of leaves, on the photochemical activity of isolated thylakoids and on the formation and spectral properties of chlorophyll-protein complexes were studied during a 72-h greening period of detached etiolated leaves of barley (Hordeum vulgare L. cv. Horpácsi kétsoros). It was established that i) the photosynthetic capacity of the leaves decreased considerably (by 80 and 90%, as determined by(14)CO2 fixation and fast fluorescence induction measurements, respectively); ii) the photochemical activity of isolated thylakoids from water to potassium ferricyanide and from dichlorophenol indophenol/ascorbate to methylviologen exhibited only slight reductions when expressed on a chlorophyll basis compared with the control; iii) the slow fluorescence induction curves of the treated leaves demonstrated the presence of a peculiar fluorescence component interrupting the quenching of fluorescence at around 1 min illumination; iv) a shortage of the chlorophyll-protein complex of photosystem I (CPI) occurred with a higher content of the monomer of the light harvesting complex in the thylakoids of treated leaves; and v) the fluorescence spectrum of the CPI band present in treated leaves indicates the destruction of the structural integrity of this complex during isolation from the membrane.
在离体黄化大麦（Hordeum vulgare L. cv. Horpácsi kétsoros）叶片的 72 小时变绿期内，研究了哒嗪酮化合物 SAN 9785 对叶片光合作用能力、分离类囊体的光化学活性以及叶绿素-蛋白质复合物的形成和光谱特性的影响。结果表明：i）叶片的光合能力显著下降（通过(14)CO2 固定和快速荧光诱导测量，分别下降了 80%和 90%）；ii）与对照相比，用叶绿素标准化时，从水到铁氰化钾以及从二氯酚靛酚/抗坏血酸到甲紫精的分离类囊体的光化学活性仅略有降低；iii）处理叶片的缓慢荧光诱导曲线显示存在一种特殊的荧光成分，在 1 分钟光照时中断荧光猝灭；iv）在处理叶片的类囊体中，出现了光系统 I（CPI）叶绿素-蛋白质复合物的短缺，同时单体的含量较高；v）在处理叶片中存在的 CPI 带的荧光光谱表明，在从膜中分离时，该复合物的结构完整性遭到破坏。
