12 - O - 十四酰佛波醇 - 13 - 乙酸酯（TPA）对培养的人表皮角质形成细胞磷脂代谢的影响。

The effect of 12-O-tetradecanoylphorbol-13-acetate (TPA) on phospholipid metabolism of human epidermal keratinocytes in culture.

作者信息

DeLeo V A, Scheide S, Horlich H, Hanson D, Southern W, Harber L C, Weinstein I B

出版信息

Carcinogenesis. 1986 Jun;7(6):937-42. doi: 10.1093/carcin/7.6.937.

DOI:10.1093/carcin/7.6.937

Abstract

The tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) stimulated the release of [3H]choline from prelabelled membrane phospholipids of cultured keratinocytes obtained from normal human skin. In contrast, TPA in the concentration range of 10(-12) to 10(-6) g/ml failed to induce deacylation of [3H]arachidonic acid or stimulate [3H]prostaglandin production in prelabelled keratinocytes. In addition, TPA did not induce [3H]choline incorporation into the membrane phospholipids of these cells. The previously reported inability of TPA to stimulate a proliferative response in these cell cultures may be related to the resistance of these cells to TPA-induced alterations of arachidonate metabolism.

摘要

肿瘤促进剂12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）刺激了从正常人皮肤获得的培养角质形成细胞预先标记的膜磷脂中[3H]胆碱的释放。相比之下，浓度范围为10(-12)至10(-6) g/ml的TPA未能诱导预先标记的角质形成细胞中[3H]花生四烯酸的脱酰作用，也未刺激[3H]前列腺素的产生。此外，TPA并未诱导[3H]胆碱掺入这些细胞的膜磷脂中。先前报道的TPA在这些细胞培养物中无法刺激增殖反应，可能与这些细胞对TPA诱导的花生四烯酸代谢改变的抗性有关。