新制癌菌素发色团的不稳定性。

Unstability of neocarzinostatin-chromophore.

作者信息

Edo K, Sato H, Saito K, Akiyama Y, Kato M, Mizugaki M, Koide Y, Ishida N

出版信息

J Antibiot (Tokyo). 1986 Apr;39(4):535-40. doi: 10.7164/antibiotics.39.535.

DOI:10.7164/antibiotics.39.535

Abstract

The unstability of neocarzinostatin (NCS), apo-NCS and NCS-chromophore (NCS-chr) has been investigated by using an extra-weak chemiluminescence (CL) analyzer. A significantly high emission intensity (10,840 counts/10 seconds) was detected from NCS under dark conditions at 20 degrees C, while no significant emission was observed in other antitumor antibiotics, such as, mitomycin C and pepleomycin. This high emission intensity of NCS was due to NCS-chr I (epoxide form) but not apo-NCS. The functional group generating the high extra-weak CL of NCS-chr I is probably the epoxide in the molecule, since the emission intensity of NCS-chr I (epoxide form) is much higher than that of NCS-chr II (hydrochloride adduct form). The extra-weak CL emission of NCS decreased under a nitrogen atmosphere and it was greatly enhanced under an oxygen atmosphere. The spectral analysis of NCS showed emission peaks around 460 and 570 nm. These observations strongly suggest that one of the emission species of NCS-chr may be due to singlet oxygen.

摘要

利用超微弱化学发光（CL）分析仪研究了新制癌菌素（NCS）、脱辅基NCS和NCS发色团（NCS-chr）的不稳定性。在20℃黑暗条件下，从NCS检测到显著高的发射强度（10840计数/10秒），而在其他抗肿瘤抗生素如丝裂霉素C和培洛霉素中未观察到显著发射。NCS的这种高发射强度归因于NCS-chr I（环氧化物形式）而非脱辅基NCS。产生NCS-chr I高超微弱CL的官能团可能是分子中的环氧化物，因为NCS-chr I（环氧化物形式）的发射强度远高于NCS-chr II（盐酸加合物形式）。NCS的超微弱CL发射在氮气气氛下降低，在氧气气氛下大大增强。NCS的光谱分析显示在460和570nm左右有发射峰。这些观察结果有力地表明，NCS-chr的发射物种之一可能是单线态氧。