Sterling P, Lampson L A
J Neurosci. 1986 May;6(5):1314-24. doi: 10.1523/JNEUROSCI.06-05-01314.1986.
The inner plexiform layer of cat retina contains synaptic structures belonging to 50 or more types of "identified" neurons. To learn whether there are antigens confined to subsets of these synaptic structures, we raised monoclonal antibodies to homogenates of neural retina. Binding patterns of these antibodies were visualized by the peroxidase-antiperoxidase method and studied in serial, ultrathin sections by electron microscopy. Four antibodies stained the synaptic varicosities of certain amacrine cells. Many of the stained varicosities formed reciprocal synapses with a rod bipolar axon terminal, but only about half of the reciprocal synapses associated with a rod bipolar were stained. Other stained varicosities formed synapses with cone bipolar axons, ganglion cell dendrites, and unstained amacrine processes. The patterns were essentially the same for each antibody and were not altered by staining with the antibodies two at a time; therefore, it is likely that all four antibodies stain the same subset of synaptic structures. These patterns would be accounted for if there were staining of all the synaptic varicosities of three of the four types of identified amacrine reciprocally connected to the rod bipolar (A6, A8, A13). This localization suggests that the antigen responsible for the binding pattern is not associated with synaptic transmission. Staining is present in the inner plexiform layer during the period of synaptogenesis and consequently the antibodies are serving as markers for following the development of identified synapses in an identified neural circuit.
猫视网膜的内网层包含属于50多种“已鉴定”神经元的突触结构。为了了解是否存在局限于这些突触结构亚群的抗原，我们制备了针对神经视网膜匀浆的单克隆抗体。这些抗体的结合模式通过过氧化物酶-抗过氧化物酶方法进行可视化，并通过电子显微镜在连续超薄切片中进行研究。四种抗体对某些无长突细胞的突触膨体进行了染色。许多被染色的膨体与视杆双极轴突终末形成相互突触，但与视杆双极相关的相互突触中只有约一半被染色。其他被染色的膨体与视锥双极轴突、神经节细胞树突和未染色的无长突细胞突起形成突触。每种抗体的模式基本相同，并且一次用两种抗体染色不会改变；因此，很可能所有四种抗体都对同一突触结构亚群进行了染色。如果与视杆双极（A6、A8、A13）相互连接的四种已鉴定无长突细胞中的三种的所有突触膨体都被染色，那么这些模式就可以得到解释。这种定位表明负责结合模式的抗原与突触传递无关。在突触发生期间，内网层中存在染色，因此这些抗体可作为追踪特定神经回路中特定突触发育的标记物。
