Rogers C S, Morse D E
J Submicrosc Cytol. 1986 Apr;18(2):313-24.
Light microscopy and histochemistry consisting of alcian blue staining were utilized to study atrial septation in the albino rat heart. Light microscopy shows septum primum to develop from the dorsocranial wall of the primitive atrium by day 12.50. Foramen secundum rapidly develops in the ventrocranial portion of septum primum during day 13.00. A second septum appears to the right of septum primum in the rat as the foramen secundum increases in size. The first evidence of septum secundum can be noted on histologic preparations as a small rounded bulge extending into the right atrium from the ventrocranial wall. This occurs at day 14.50. This septum continues to enlarge but does not fuse with the endocardial cushions as does septum primum. Alcian blue staining shows the presence of glycosaminoglycans (GAGs) concentrated heavily in the leading edge of the 12.50 day septum primum. GAGs are also present during foramen secundum formation in areas where endocardial cells aggregate. As development progresses, GAGs diminish in all areas of septum primum. Of particular interest is an area of intense alcian blue staining in the wall of the right atrium ventral and caudal to septum secundum development. This appears in histologic preparations of 13.50 and 14.50 day rat hearts.
利用光学显微镜和包括阿尔新蓝染色在内的组织化学方法，研究白化大鼠心脏的房间隔形成过程。光学显微镜显示，在第12.50天时，原发隔从原始心房的背颅壁开始发育。在第13.00天时，继发孔在原发隔的腹颅部分迅速形成。随着继发孔尺寸增大，大鼠体内在原发隔右侧出现第二个隔膜。在组织学切片上，继发隔最早的迹象表现为一个小的圆形凸起，从腹颅壁延伸至右心房。这一现象发生在第14.50天。这个隔膜持续增大，但不像原发隔那样与心内膜垫融合。阿尔新蓝染色显示，糖胺聚糖（GAGs）大量集中在第12.50天的原发隔前缘。在继发孔形成过程中，心内膜细胞聚集的区域也存在GAGs。随着发育进程推进，原发隔所有区域的GAGs含量都减少。特别值得关注 的是，在继发隔发育部位腹侧和尾侧的右心房壁上，有一个阿尔新蓝染色强烈的区域。这出现在第13.50天和第14.50天大鼠心脏的组织学切片中。
