Jarow J P, Marshall F F, Isaacs J T
J Urol. 1986 Jun;135(6):1312-8. doi: 10.1016/s0022-5347(17)46082-4.
A biological assay using the chorioallantoic membrane (CAM) of the chicken egg as an in vitro culture system has been developed and standardized in order to assess the androgen responsiveness of normal and abnormal prostatic tissue. In this assay, test tissue is implanted onto the CAM of chicken eggs in the presence or absence of exogenous testosterone treatment, with subsequent serum levels of 45 ng./dl. and greater than 5,000 ng./dl., respectively. The responsiveness of test tissue to low versus high androgen levels was evaluated in this CAM assay using both cellular morphology and mitotic index as response criteria. Both androgen responsive and unresponsive tissues from the rat were grown on the CAM and demonstrated appropriate morphologic and proliferative responses to the presence and absence of testosterone supplementation. Human prostatic adenocarcinoma and benign hyperplastic tissues were also grown successfully on the CAM. Documentation of human tissue survival was performed by immunocytochemical analysis for prostate specific antigen and prostate specific acid phosphatase. The human prostatic adenocarcinomas studied demonstrated a two to four-fold increase in mitotic index with androgen augmentation. In conclusion, the ability of this assay to determine androgen responsiveness as measured by morphologic and proliferative criteria has been documented in rat tissues. Human prostatic adenocarcinoma has been grown consistently on the chick chorioallantoic membrane for the first time using newly developed techniques. Therefore, this technique shows promise in the clinical application for the determination of the androgen responsiveness of human prostatic carcinomas.
为了评估正常和异常前列腺组织的雄激素反应性，已开发并标准化了一种使用鸡胚绒毛尿囊膜（CAM）作为体外培养系统的生物学检测方法。在该检测中，将测试组织植入鸡胚的CAM上，分别在有或没有外源性睾酮处理的情况下，随后血清水平分别为45 ng./dl和大于5000 ng./dl。在该CAM检测中，使用细胞形态和有丝分裂指数作为反应标准，评估测试组织对低雄激素水平与高雄激素水平的反应性。来自大鼠的雄激素反应性和无反应性组织都在CAM上生长，并对补充和不补充睾酮表现出适当的形态学和增殖反应。人前列腺腺癌和良性增生组织也成功地在CAM上生长。通过对前列腺特异性抗原和前列腺特异性酸性磷酸酶进行免疫细胞化学分析来记录人体组织的存活情况。所研究的人前列腺腺癌在雄激素增加时，有丝分裂指数增加了两到四倍。总之，该检测方法通过形态学和增殖标准来确定雄激素反应性的能力已在大鼠组织中得到证实。使用新开发的技术，人前列腺腺癌首次在鸡胚绒毛尿囊膜上持续生长。因此，该技术在临床应用中显示出有望用于确定人前列腺癌的雄激素反应性。
