Pokudin N I, Orlov S N, Postnov Iu V
Kardiologiia. 1986 Mar;26(3):94-9.
A study of Ca-ATPase and Ca-pump activity of the erythrocyte membrane's inverted vesicles with different cytoskeletal protein status demonstrated that Ca-pump activity remained unchanged, and Ca-ATPase activity was increased by 20-40%, as compared to erythrocytes from the normotensive controls, in hypertensive patients in the absence of exogenous calmodulin. These differences are assumed to be due to disorders of erythrocyte membrane structure in primary hypertension resulting in less efficient Ca-pump operation in the inverted vesicles. In all types of membranes obtained from hypertensive patients' erythrocytes in conditions of iso-osmotic hemolysis, calmodulin-induced increment of peak Ca-ATPase and Ca-pump activity was 2 or 3 times as low as that of normotensive controls. These differences remained in evidence after the solubilization of membrane proteins with triton X-100. No differences in calmodulin influence on Ca-ATPase affinity to Ca2+ could be found. The membranes obtained in iso-osmotic conditions demonstrated no calmodulin effect on Ca-ATPase activity which is attributed to partial proteolysis of the enzyme's molecule.
一项关于不同细胞骨架蛋白状态的红细胞膜内翻囊泡的钙 - ATP酶和钙泵活性的研究表明，在没有外源性钙调蛋白的情况下，与血压正常对照组的红细胞相比，高血压患者的钙泵活性保持不变，而钙 - ATP酶活性增加了20 - 40%。这些差异被认为是由于原发性高血压中红细胞膜结构紊乱导致内翻囊泡中钙泵运作效率降低。在等渗溶血条件下从高血压患者红细胞获得的所有类型膜中，钙调蛋白诱导的钙 - ATP酶和钙泵活性峰值的增加比血压正常对照组低2至3倍。在用曲拉通X - 100溶解膜蛋白后，这些差异仍然存在。未发现钙调蛋白对钙 - ATP酶与Ca2+亲和力的影响有差异。在等渗条件下获得的膜显示钙调蛋白对钙 - ATP酶活性没有影响，这归因于该酶分子的部分蛋白水解。
