Scheit K H, Zimmer M, Rao N S, Prasad K S, Bhargava P M
Microbios. 1986;45(183):105-12.
Seminalplasmin, a 6,000 dalton antimicrobial protein present in bovine seminal plasma, is shown to inhibit growth and/or RNA synthesis in several bacterial species. In only one strain out of twenty one belonging to fourteen species, did both RNA synthesis and growth appear to be resistant to seminalplasmin. The antibacterial activity of seminalplasmin, in the case of E. coli, was also studied as a function of its concentration and of time; the minimal concentration of the protein required for 100% bactericidal activity was only about twice that required for 100% bacteriostatic activity. The killing of E. coli cells proceeded in two phases, a slow phase and then a rapid one, and required several hours for completion. Several bacterial species tested secreted proteases into the medium that destroyed seminalplasmin.
精浆蛋白是一种存在于牛精浆中的6000道尔顿抗菌蛋白，已证明它能抑制多种细菌的生长和/或RNA合成。在属于14个物种的21个菌株中，只有一个菌株的RNA合成和生长似乎对精浆蛋白具有抗性。对于大肠杆菌，还研究了精浆蛋白的抗菌活性与其浓度和时间的关系；实现100%杀菌活性所需的蛋白质最低浓度仅约为实现100%抑菌活性所需浓度的两倍。大肠杆菌细胞的杀灭过程分为两个阶段，一个缓慢阶段，然后是一个快速阶段，并且需要几个小时才能完成。测试的几种细菌物种会向培养基中分泌蛋白酶，这些蛋白酶会破坏精浆蛋白。
