Veselovskiĭ N S, Fomina A F
Neirofiziologiia. 1986;18(2):207-14.
Sodium and calcium channels passing inward currents were studied by intracellular dialysis technique and voltage clamp in the somatic membrane of neuroblastoma cells during their morphological differentiation induced by increasing pH of the culture medium up to 8.0-8.2. Kinetic and voltage-dependent properties of sodium and calcium channels of differentiated cells and cells grown in the suspension culture were identical. Densities of sodium currents in the somatic membrane of neuroblastoma cells grown in suspension culture were about 7.3 +/- 0.8 microA/microF and from 37 +/- 5.2 microA/microF to 54.7 +/- 3.6 microA/microF of differentiated cells at different terms of cultivation. Densities of calcium currents in the membrane of cells grown in suspension were about 1.4 +/- 0.2 microA/microF, while in differentiated cells they ranged from 1.1 +/- 0.2 to 2.8 +/- 0.4 microA/microF at different terms of cultivation. Induction or reduction of differentiation by varying pH of the culture medium produced reciprocal changes in densities of sodium and calcium channels.
在将培养基pH值提高到8.0 - 8.2诱导神经母细胞瘤细胞进行形态分化的过程中，运用细胞内透析技术和电压钳技术，对通过内向电流的钠通道和钙通道进行了研究。分化细胞与悬浮培养细胞的钠通道和钙通道的动力学及电压依赖性特性是相同的。悬浮培养的神经母细胞瘤细胞体膜上钠电流密度约为7.3±0.8微安/微法，在不同培养时期，分化细胞的钠电流密度为37±5.2微安/微法至54.7±3.6微安/微法。悬浮培养细胞的细胞膜上钙电流密度约为1.4±0.2微安/微法，而在不同培养时期，分化细胞的钙电流密度范围为1.1±0.2至2.8±0.4微安/微法。通过改变培养基pH值诱导或减少分化，会使钠通道和钙通道密度产生相反的变化。
