Department of Clinical laboratory, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Department of Mammary Gland and Gynecologic Oncology, Guizhou Cancer Hospital, Guiyang, China.
Biomed Pharmacother. 2014 Apr;68(3):291-7. doi: 10.1016/j.biopha.2013.10.004. Epub 2013 Oct 18.
Cervical cancer is one of the most common gynecologic tumors. There is an upward trend in the incidence. The objective of this research was to explore the effect of lobaplatin on cervical cancer CaSki cells proliferation, cell cycle and apoptosis and analysis of the differential expressed proteins of CaSki cells after exposed to lobaplatin. Our findings have shown that lobaplatin inhibits cell proliferations in human cervical cancer CaSki cells in dose- and time-dependent manner. Flow cytometry assay confirmed that lobaplatin affected cervical cancer cell survival by blocking cell cycle progression in S phase and G0/G1 phase and inducing apoptosis in dose- and time-dependent manner. Lobaplatin treatment reduced polypyrimidine tract-binding protein 2, ribose-phosphate pyrophosphokinase, hypothetical protein, terminal uridylyltransferase 7, ubiquitin specific protease 16 and heterogeneous nuclear ribonucleoprotein A2/B1 expression and increase zinc finger protein 91, zinc finger protein, C-X-C motif chemokine 10 precursor, stromal cell protein and laminin subunit alpha-4 expression. Some of the differentially expressed proteins may be associated with antitumor effect of lobaplatin. Lobaplatin showed a good antitumour activity in in vitro models of human cervical cancer cells. These results indicate that lobaplatin could be an effective chemotherapeutic agent in human cervical cancer treatment by inducing apoptosis, cell cycle arrest and changing many kinds of protein molecule expression level.
宫颈癌是最常见的妇科肿瘤之一。其发病率呈上升趋势。本研究旨在探讨洛铂对宫颈癌 CaSki 细胞增殖、细胞周期和凋亡的影响，并分析 CaSki 细胞暴露于洛铂后的差异表达蛋白。我们的研究结果表明，洛铂呈剂量和时间依赖性抑制人宫颈癌 CaSki 细胞的增殖。流式细胞术检测证实，洛铂通过阻滞 S 期和 G0/G1 期细胞周期进程和诱导细胞凋亡，呈剂量和时间依赖性抑制宫颈癌细胞的存活。洛铂处理降低多嘧啶核苷酸结合蛋白 2、核糖磷酸焦磷酸激酶、假定蛋白、末端尿嘧啶核苷转移酶 7、泛素特异性蛋白酶 16 和异质核核糖核蛋白 A2/B1 的表达，增加锌指蛋白 91、锌指蛋白、C-X-C 基元趋化因子 10 前体、基质细胞蛋白和层粘连蛋白亚单位α-4 的表达。其中一些差异表达蛋白可能与洛铂的抗肿瘤作用有关。洛铂在体外人宫颈癌模型中表现出良好的抗肿瘤活性。这些结果表明，洛铂通过诱导细胞凋亡、细胞周期阻滞和改变多种蛋白分子的表达水平，可能成为治疗宫颈癌的有效化疗药物。
