Botanisches Institut der Westfälischen Wilhelms-Universität, Schloßgarten 3, D-4400, Münster, Federal Republic of Germany.
Planta. 1986 Apr;167(4):444-51. doi: 10.1007/BF00391219.
Antisera were raised against two forms of α-1,4-glucan phosphorylase (EC 2.4.1.1) which had been purified from leaves of Spinacia oleracea L. Immunoglobulin G preparations were isolated from the antisera, and their specificity was ensured by immunoplobulin G preparations were used for in situ localization of the two phosphorylase forms in spinach leaf thin sections by indirect immuno-fluorescence. Both enzyme forms were present in the palisade and spongy parenchyma and in the guard cells, but their intracellular distribution was complementary. One phosphorylase form (designated as the chloroplastic form) was restricted to the stromal space of chloroplasts whereas the other (the non-chloroplastic form) was present only in the cytoplasm of chloroplast-containing cells. Thus, the phosphorylases represent two distinct compartment-specific enzyme forms which reside within the same photosynthetically active mesophyll cell.
抗血清是针对两种形式的α-1,4-葡聚糖磷酸化酶（EC 2.4.1.1）从菠菜叶中纯化而产生的。从抗血清中分离出免疫球蛋白 G 制剂，并通过间接免疫荧光法使用免疫球蛋白 G 制剂在菠菜叶薄片中对两种磷酸化酶形式进行原位定位，以确保其特异性。两种酶形式均存在于栅栏组织和海绵组织以及保卫细胞中，但它们的细胞内分布是互补的。一种磷酸化酶形式（称为叶绿体形式）仅限于叶绿体的基质空间，而另一种（非叶绿体形式）仅存在于含有叶绿体的细胞的细胞质中。因此，磷酸化酶代表两种不同的区室特异性酶形式，它们存在于同一光合作用活跃的叶肉细胞内。
