Thelin S, Hultman J, Ronquist G, Hansson H E
Thorac Cardiovasc Surg. 1986 Apr;34(2):104-9. doi: 10.1055/s-2007-1020388.
The paracorporeal rat heart model was used to investigate the extent of myocardial protection by potassium cardioplegia supplemented with phosphoenolpyruvate (PEP) (14.4 mM) and ATP (0.067 mM) singly or in combination. Rat hearts were subjected to 30 minutes of ischemia at 37 degrees. They were subsequently reperfused for 40 minutes during which time the left ventricular isovolumic work was measured and blood samples were taken for creatine kinase isoenzyme MB (CK-MB) analysis. At the end of each experiment the hearts were freeze-clamped for later analyses of high energy phosphate compounds. Supplementation with PEP and ATP (Group I) and with only ATP (Group II) showed a significantly better left ventricular isovolumic work and a significantly higher adenylate charge potential (ACP). Supplementation with only PEP (Group III) resulted in significantly better left ventricular isovolumic work than the control group but significantly lower than groups I and II. There were no significant differences between the groups in regard to the CK-MB efflux. Supplementation with PEP and ATP in combination did not show any positive effect at 40 minutes of reperfusion over and above that which was achieved with ATP only.
采用体外大鼠心脏模型，研究单独或联合添加磷酸烯醇丙酮酸（PEP，14.4 mM）和三磷酸腺苷（ATP，0.067 mM）的钾停搏液对心肌的保护程度。将大鼠心脏在37℃下进行30分钟的缺血处理。随后进行40分钟的再灌注，在此期间测量左心室等容功，并采集血样进行肌酸激酶同工酶MB（CK-MB）分析。在每个实验结束时，将心脏冷冻钳夹，以备后续分析高能磷酸化合物。添加PEP和ATP（I组）以及仅添加ATP（II组）的处理显示左心室等容功明显更好，腺苷酸电荷电位（ACP）明显更高。仅添加PEP（III组）导致左心室等容功明显优于对照组，但明显低于I组和II组。各组之间在CK-MB流出方面无显著差异。在再灌注40分钟时，联合添加PEP和ATP并未显示出比仅添加ATP有任何额外的积极效果。
