Windsor R S
Res Vet Sci. 1978 Jan;24(1):109-12.
The T1 broth vaccine against contagious bovine pleuropneumonia can only be stored for four weeks at +4 degrees C because after that time the titre of Mycoplasma mycoides sub-species mycoides falls below the minimal vaccinating dose. An investigation into the death of these organisms during the stationary growth phase was made. The culture medium in which the vaccine was prepared was found to contain adequate nutrients. Control of the pH by the addition of KOH was shown to preserve the viability of the organism: there was no drop in titre during 336 h (14 days) at 37 degrees C. Use of this technique may prove valuable as a means of preserving the viability of T1 broth vaccine.
用于防治牛传染性胸膜肺炎的T1肉汤疫苗在4摄氏度下只能保存四周，因为在这段时间之后，丝状支原体丝状亚种的滴度会降至最低接种剂量以下。针对这些生物体在稳定生长期死亡的情况进行了调查。发现制备疫苗所用的培养基含有充足的营养成分。通过添加氢氧化钾来控制pH值，结果表明可保持生物体的活力：在37摄氏度下336小时（14天）内滴度没有下降。使用该技术作为保存T1肉汤疫苗活力的一种方法可能证明是有价值的。
