利用噬菌体作为平台筛选癌细胞靶向肽。
Using phage as a platform to select cancer cell-targeting peptides.
作者信息
Li Xin, Mao Chuanbin
机构信息
Department of Chemistry & Biochemistry, Stephenson Life Sciences Center, University of Oklahoma, Norman, OK, USA.
出版信息
Methods Mol Biol. 2014;1108:57-68. doi: 10.1007/978-1-62703-751-8_4.
Abstract
One challenge in the development of cancer therapies is the availability of cancer-specific ligands. Recently, phage-displayed peptide libraries have been used for the selection of peptide-based cell-targeting ligands, especially cancer cell ligands. Here we describe the methods to identify SKBR-3 breast cancer cell-specific peptides from a phage-displayed random peptide library. It is possible to select both cell-surface-binding and cell-internalizing peptides using this method. This method can also be applied to the selection of targeting peptides for other adherent cancer cells. The identified short peptides can be potentially incorporated into a variety of early diagnostic and targeted therapeutic systems against breast cancer.
摘要
癌症治疗发展中的一个挑战是癌症特异性配体的可用性。最近，噬菌体展示肽库已被用于筛选基于肽的细胞靶向配体，尤其是癌细胞配体。在此，我们描述了从噬菌体展示随机肽库中鉴定SKBR-3乳腺癌细胞特异性肽的方法。使用该方法可以筛选出细胞表面结合肽和细胞内化肽。该方法也可应用于其他贴壁癌细胞靶向肽的筛选。所鉴定的短肽有可能被整合到多种针对乳腺癌的早期诊断和靶向治疗系统中。
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