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十二烷基硫酸钠-聚丙烯酰胺凝胶电泳后，通过从染色条带中洗脱考马斯亮蓝R来定量蛋白质。

Quantitation of proteins by elution of Coomassie brilliant blue R from stained bands after sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

作者信息

Ball E H

出版信息

Anal Biochem. 1986 May 15;155(1):23-7. doi: 10.1016/0003-2697(86)90218-6.

DOI:10.1016/0003-2697(86)90218-6
PMID:2424336
Abstract

A simple method for the extraction of Coomassie brilliant blue R from stained protein bands excised from polyacrylamide gels is described. Spectrophotometric measurement of the eluted dye forms the basis of a sensitive assay to quantitate proteins in gels in the range 0.5-10 micrograms. The method requires no unusual equipment and is suitable for measurement of multiple samples. The polypeptide is not extracted and remains available for further analysis. The technique has been applied to three proteins and gels of various acrylamide percentages.

摘要

本文描述了一种从聚丙烯酰胺凝胶中切下的经染色的蛋白质条带中提取考马斯亮蓝R的简单方法。对洗脱染料进行分光光度测量，构成了一种灵敏测定法的基础，可对凝胶中0.5 - 10微克范围内的蛋白质进行定量。该方法不需要特殊设备，适用于多个样品的测量。多肽不会被提取出来，仍可用于进一步分析。该技术已应用于三种蛋白质以及不同丙烯酰胺百分比的凝胶。

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