一种提高人血浆中低水平丙戊酸测定灵敏度的新衍生化方法。
A new derivatization method to enhance sensitivity for the determination of low levels of valproic acid in human plasma.
作者信息
Zhang Jin-Feng, Zhang Zhi-Qing, Dong Wei-Chong, Jiang Ye
机构信息
Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang, Hebei Province 050017, China.
Department of Pharmacy, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei Province 050000, China.
出版信息
J Chromatogr Sci. 2014 Nov-Dec;52(10):1173-80. doi: 10.1093/chromsci/bmt167. Epub 2013 Nov 15.
A novel and sensitive high-performance liquid chromatographic (HPLC) method has been developed and validated for the determination of valproic acid (VPA) in human plasma. The method was based on derivatization of VPA using 2-bromo-2'-acetonaphthone as a new derivatization reagent. Caprylic acid was used as an internal standard (IS). Under the optimized extraction and derivatization conditions, the method showed good linearity in the range of 0.05-200 μg mL(-1) and the limit of detection was as low as 0.01 μg mL(-1). The relative standard deviation for intra-day and inter-day (n = 5) was <5%. The recovery ranged from 95.2 to 101.4%. The proposed method is proved to be highly sensitive, simple and rapid, and was successfully applied to the analysis of VPA in plasma samples from patients with generalized epilepsy.
已开发并验证了一种新颖且灵敏的高效液相色谱（HPLC）方法，用于测定人血浆中的丙戊酸（VPA）。该方法基于使用2-溴-2'-乙酰萘作为新型衍生化试剂对VPA进行衍生化。辛酸用作内标（IS）。在优化的萃取和衍生化条件下，该方法在0.05 - 200 μg mL(-1)范围内显示出良好的线性，检测限低至0.01 μg mL(-1)。日内和日间（n = 5）的相对标准偏差<5%。回收率在95.2%至101.4%之间。所提出的方法被证明具有高灵敏度、简单快速，并成功应用于全身性癫痫患者血浆样本中VPA的分析。
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