Honorary Maeda-Duffey Laboratory, University of California Davis, Davis, California, United States of America.
PLoS One. 2013 Nov 14;8(11):e80182. doi: 10.1371/journal.pone.0080182. eCollection 2013.
A three-step, quasi-double-bind approach was used as a proof-of-concept study to screen twenty compounds for their ability to reduce oviposition of gravid female navel orangeworm(NOW), Ameylois transitella (Lepidoptera: Pyralidae). First, the panel of compounds, whose identity was unknown to the experimenters, was tested by electroantennogram (EAG) using antennae of two-day old gravid females as the sensing element. Of the twenty compounds tested three showed significant EAG responses. These three EAG-active compounds and a negative control were then analyzed for their ability to reduce oviposition via small-cage, two-choice laboratory assays. Two of the three compounds significantly reduced oviposition under laboratory conditions. Lastly, these two compounds were deployed in a field setting in an organic almond orchard in Arbuckle, CA using black egg traps to monitor NOW oviposition. One of these two compounds significantly reduced oviposition on black egg traps under these field conditions. Compound 9 (later identified as isophorone) showed a significant reduction in oviposition in field assays and thus has a potential as a tool to control the navel orangeworm as a pest of almonds.
采用三步准双盲法作为概念验证研究，筛选 20 种化合物，以评估其对怀有身孕的棉铃象甲（NOW）（鳞翅目：麦蛾科）雌虫产卵的抑制能力。首先，用触角作为感应元件，对实验者未知身份的化合物组进行电触角图谱（EAG）测试。在测试的 20 种化合物中，有 3 种表现出显著的 EAG 反应。然后，对这 3 种 EAG 活性化合物和 1 种阴性对照物进行分析，以评估它们在小 cage、双选择实验室测定中减少产卵的能力。这 3 种化合物中的 2 种在实验室条件下显著降低了产卵。最后，这两种化合物在加利福尼亚州阿巴克尔的有机杏仁果园中使用黑色卵诱捕器进行田间试验，以监测 NOW 的产卵情况。这两种化合物中的 1 种化合物显著减少了在田间条件下黑色卵诱捕器上的产卵。化合物 9（后来鉴定为异佛尔酮）在田间试验中表现出显著的产卵减少，因此有可能作为控制杏仁象甲这种杏仁害虫的工具。
