Lee Sangdae, Kim Yong-Il, Kim Ki-Bok
National Academy of Agricultural Science, 150 Suin-Ro Kweonseonku, Suwon 441-707, Korea.
J Nanosci Nanotechnol. 2013 Nov;13(11):7319-24. doi: 10.1166/jnn.2013.8082.
Biosensors are used in a variety of fields for early diagnosis of diseases, measurement of toxic contaminants, quick detection of pathogens, and separation of specific proteins or DNA. In this study, we fabricated and evaluated the capability of a high sensitivity Love wave surface acoustic wave (SAW) biosensor. The experimental setup was composed of the fabricated 155-MHz Love wave SAW biosensor, a signal measurement system, a liquid flow system, and a temperature-control system. Subsequently, we measured the lower limit of detection (LOD) of the 155-MHz Love wave SAW biosensor, and calculated the association and dissociation constants between protein G and anti-mouse IgG using kinetic analysis. We compared these results with those obtained using a commercial surface plasmon resonance (SPR) biosensor. We found that the LOD of the SAW biosensor for anti-mouse IgG and mouse IgG was 0.5 and 1 microg/ml, respectively, and the resultant equilibrium association and dissociation constants were similar to the corresponding values obtaining using the commercial SPR biosensor. Thus, we conclude that the fabricated 155-MHz Love wave SAW biosensor exhibited the high sensitivity of the commercial SPR biosensor and was able to analyze the binding properties of the ligand and receptor by kinetic analysis similarly to the commercial SPR biosensor.
生物传感器被应用于多个领域，用于疾病的早期诊断、有毒污染物的测量、病原体的快速检测以及特定蛋白质或DNA的分离。在本研究中，我们制备并评估了一种高灵敏度的乐甫波表面声波（SAW）生物传感器的性能。实验装置由制备的155 MHz乐甫波SAW生物传感器、信号测量系统、液体流动系统和温度控制系统组成。随后，我们测量了155 MHz乐甫波SAW生物传感器的检测下限（LOD），并使用动力学分析计算了蛋白G与抗小鼠IgG之间的结合和解离常数。我们将这些结果与使用商业表面等离子体共振（SPR）生物传感器获得的结果进行了比较。我们发现，SAW生物传感器对抗小鼠IgG和小鼠IgG的LOD分别为0.5和1微克/毫升，所得的平衡结合和解离常数与使用商业SPR生物传感器获得的相应值相似。因此，我们得出结论，制备的155 MHz乐甫波SAW生物传感器表现出与商业SPR生物传感器相同的高灵敏度，并且能够像商业SPR生物传感器一样通过动力学分析来分析配体和受体的结合特性。
