de Oliveira Pollyanna Francielli, de Andrade Kelly Jacqueline Barbosa, Paula Marcela Cristina Ferreira, Oliveira Acésio Nathália, da Silva Moraes Thais, Borges Priscilla Scalon Freitas, Barcelos Gustavo Rafael Mazzaron, Tavares Denise Crispim
Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201 - Parque Universitário, 14404-600 Franca, São Paulo, Brazil.
Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Avenida Café s/n, 14040-903 Ribeirão Preto, São Paulo, Brazil.
Mutat Res Genet Toxicol Environ Mutagen. 2014 Jan 1;759:37-42. doi: 10.1016/j.mrgentox.2013.07.017. Epub 2013 Nov 15.
Bixin is a carotenoid found in the seeds of Bixa orellana L., a plant native to tropical America that is used in the food industry. The aim of this study was to investigate the effect of bixin on DNA damage and pre-neoplastic lesions induced by 1,2-dimethylhydrazine (DMH) in the liver and colon of Wistar rats. The animals received bixin at daily doses of 0.1, 1.0 and 10mg/kg body weight (bw) by gavage. For the assessment of DNA damage in hepatocytes and colon cells with the comet assay, the administration of bixin was for 7 days. The animals received a single subcutaneous injection of 25mg/kg bw of DMH, and were euthanized 4h later. For the evaluation of the frequency of aberrant crypt foci (ACF), the animals were treated with the different doses of bixin for 4 weeks. Four doses of 40mg/kg bw DMH, two doses in the first week and two doses in the second week, were administered and euthanasia occurred at 4 weeks after the beginning of treatment. Bixin reduced the frequency of DNA damage in hepatocytes at the highest two doses tested (1.0 and 10mg/kg bw). On the other hand, no differences in the frequency of DNA damage in colon cells were observed between animals treated with bixin plus DMH and those treated with DMH alone. In addition, the frequency of ACF did not differ significantly between the group treated with bixin plus DMH and the DMH group. The results suggest that bixin does not suppress the formation of ACF, indicating the absence of a protective effect against colon carcinogenesis.
胭脂树素是一种类胡萝卜素，存在于红木（Bixa orellana L.）的种子中，红木是一种原产于热带美洲的植物，可用于食品工业。本研究的目的是调查胭脂树素对1,2 - 二甲基肼（DMH）诱导的Wistar大鼠肝脏和结肠DNA损伤及癌前病变的影响。动物通过灌胃接受剂量为0.1、1.0和10mg/kg体重（bw）的胭脂树素。为了用彗星试验评估肝细胞和结肠细胞中的DNA损伤，胭脂树素给药7天。动物接受一次25mg/kg bw的DMH皮下注射，并在4小时后安乐死。为了评估异常隐窝灶（ACF）的频率，动物用不同剂量的胭脂树素治疗4周。给予四剂40mg/kg bw的DMH，第一周两剂，第二周两剂，在治疗开始后4周进行安乐死。在测试的最高两剂（1.0和10mg/kg bw）下，胭脂树素降低了肝细胞中DNA损伤的频率。另一方面，在接受胭脂树素加DMH治疗的动物和仅接受DMH治疗的动物之间，未观察到结肠细胞中DNA损伤频率的差异。此外，在接受胭脂树素加DMH治疗的组和DMH组之间，ACF的频率没有显著差异。结果表明，胭脂树素不能抑制ACF的形成，表明其对结肠癌发生没有保护作用。
