Max-Planck-Institut für Züchtungsforschung, D-5000, Köln-Vogelsang, Federal Republic of Germany.
Theor Appl Genet. 1985 Dec;71(3):500-5. doi: 10.1007/BF00251196.
The many reports of phenotypic variation among plants regenerated from tissue culture suggest underlying alterations at the DNA level. This hypothesis was tested with protoplast-derived Solanum tuberosum plants. Random potato-DNA clones were used to probe the genome of individual plants at specific sites. Two out of twelve plants were shown to be variant by Southern-hybridisation with one of the tester-clones. As this clone turned out to represent 25S-rDNA, both somaclonal variants can be regarded as mutants deficient in ribosomal RNA-genes.
组织培养再生植株的表型变异的诸多报道表明，在 DNA 水平上存在潜在的改变。利用原生质体衍生的马铃薯植株对此假说进行了检验。随机的马铃薯 DNA 克隆被用于探测特定部位单个植株的基因组。Southern 杂交试验表明，12 株植物中有 2 株发生了变异，其中 1 株与测试克隆之一发生了杂交。由于该克隆代表 25S-rDNA，因此这两个体细胞变异体均可被视为核糖体 RNA 基因缺失的突变体。
