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玉米叶绿体的核糖体RNA操纵子:23S核糖体DNA的核苷酸序列及其与大肠杆菌23S核糖体DNA的同源性。

The rRNA operon from Zea mays chloroplasts: nucleotide sequence of 23S rDNA and its homology with E.coli 23S rDNA.

作者信息

Edwards K, Kössel H

出版信息

Nucleic Acids Res. 1981 Jun 25;9(12):2853-69. doi: 10.1093/nar/9.12.2853.

Abstract

The nucleotide sequence of 23S rDNA from Zea mays chloroplasts has been determined. Alignment with 23S rDNA from E.coli reveals 71 percent homology when maize 4.5S rDNA is included as an equivalent of the 3' end of E.coli 23S rDNA. Among the conserved sequences are sites for base modification. Chloramphenicol sensitivity and ribosomal subunit interaction. A proposal for the base pairs formed between 16S and 23S rRNAs during the 30S/50S subunit interaction is presented. The alignment of maize 23S rDNA with that of E.coli reveals three small insertion sequences of 25, 65 and 78 base pairs, whereas maize 16S rDNA shows only deletions when compared with the E.coli species.

摘要

已经测定了玉米叶绿体23S rDNA的核苷酸序列。当将玉米4.5S rDNA视为等同于大肠杆菌23S rDNA的3'末端时,与大肠杆菌的23S rDNA比对显示出71%的同源性。保守序列中包括碱基修饰位点、氯霉素敏感性和核糖体亚基相互作用位点。提出了在30S/50S亚基相互作用期间16S和23S rRNA之间形成的碱基对的提议。玉米23S rDNA与大肠杆菌的比对显示出三个分别为25、65和78个碱基对的小插入序列,而与大肠杆菌相比,玉米16S rDNA仅显示缺失。

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