Beretta A, Ermonval M, Larsson E L
Eur J Immunol. 1986 Jun;16(6):605-9. doi: 10.1002/eji.1830160604.
The frequency of specific cytotoxic T lymphocytes (CTL) precursors in the total CTL pool was analyzed in a lectin-driven limiting dilution system. We found that up to 10% of the expressed CTL repertoire in a normal mouse is "specific" for a given allogeneic H-2 haplotype. Split-well analysis under clonal conditions demonstrates that the antigens recognized by the effector CTL are H-2 encoded. A high frequency of CTL "specific" for self-H-2 antigens was revealed in all the experiments, accounting for about 1% of the total inducible pool of CTL. These results suggest a high degree of degeneracy of H-2 recognition by CTL and the immunocompetence of self-H-2-reactive CTL precursors in normal individuals.
在凝集素驱动的有限稀释系统中分析了总细胞毒性T淋巴细胞（CTL）库中特定CTL前体的频率。我们发现，正常小鼠中表达的CTL库中高达10%对给定的同种异体H-2单倍型具有“特异性”。克隆条件下的分孔分析表明，效应CTL识别的抗原是由H-2编码的。在所有实验中均发现了对自身H-2抗原“特异性”的CTL高频，约占CTL总诱导库的1%。这些结果表明CTL对H-2识别具有高度简并性，以及正常个体中自身H-2反应性CTL前体具有免疫活性。
