Biotechnology Centre, I.A.R.L., 110012, New Delhi, India.
Plant Cell Rep. 1987 Feb;6(1):43-5. doi: 10.1007/BF00269736.
Pollen plants were obtained from anther-derived calli of the indica rice variety Basmati-370. Anther-response (anthers producing pollen derived calli) and plant regeneration frequency from the pollen derived calli. was very low. Donor plants which flowered at the average max/min. temperature of 34.2°/23.3°C gave a significantly higher anther-response to in vitro techniques, than did those which flowered at 29.1°/16.4°C. Somatic callus induction and subsequent plant regeneration was readily obtained from mature seed embryos. While 2,4-D or 2,4,5-T (1 or 2 mg/l) proved highly efficient for callus induction, tryptophan (50 or 100 mg/l) induced a high frequency of green plants from the calli.
花粉植株是从籼稻品种 Basmati-370 的花药衍生愈伤组织中获得的。花药反应（产生花粉衍生愈伤组织的花药）和花粉衍生愈伤组织的植物再生频率非常低。在平均最高/最低温度为 34.2°C/23.3°C 下开花的供体植物对离体技术的花药反应明显高于在 29.1°C/16.4°C 下开花的供体植物。成熟种子胚很容易诱导体细胞愈伤组织并随后再生植物。虽然 2,4-D 或 2,4,5-T（1 或 2 mg/l）被证明对愈伤组织诱导非常有效，但色氨酸（50 或 100 mg/l）可从愈伤组织中诱导出高频的绿色植物。
