Institut für Biologie I, Auf der Morgenstelle 1, D-7400, Tübingen, Federal Republic of Germany.
Plant Cell Rep. 1987 Jun;6(3):200-3. doi: 10.1007/BF00268479.
Attempts were made to immobilize digitoxin 12β-hydroxylase, a membrane-bound, cytochrome P-450-dependent monooxygenase from cell cultures of Digitalis lanata. The optimum procedure was the entrapment of microsomes in 2% alginate by crosslinking the polysaccharide chains with CaCl2. After the immobilization of the enzyme about 70% of its activity was retained. The kinetic data such as the pH optimum and the optimum substrate concentrations were identical for the immobilized enzyme and freely suspended microsomes. Using β-methyldigitoxin as a substrate enzyme activity could be observed for more than 20 h. A continuous flow system for immobilized digitoxin 12β-hydroxylase is described.
尝试固定毛地黄 Digitoxin 12β-羟化酶，一种来源于毛地黄细胞培养物的膜结合细胞色素 P-450 依赖性单加氧酶。最佳的程序是用 CaCl2 交联多糖链，将微体包埋在 2%藻酸盐中。固定化酶后，保留了约 70%的活性。固定化酶和自由悬浮的微粒体的动力学数据，如最适 pH 值和最适底物浓度，都是相同的。使用β-甲基毛地黄毒苷作为底物，酶活性可以观察超过 20 小时。描述了一种用于固定化毛地黄毒苷 12β-羟化酶的连续流动系统。
