James J, Frederiks W M, van Noorden C J, Tas J
Histochemistry. 1986;84(4-6):308-16. doi: 10.1007/BF00482955.
Studies by means of quantitative histochemistry and cytochemistry have greatly contributed to the knowledge of metabolic changes in liver parenchymal cells. In the present paper recent work along this line is reviewed with emphasis on three topics, polyploidy as a source of metabolic heterogeneity, proteolysis in the regulation of hepatocyte cell mass and ischemic injury of hepatocytes. In all three fields, accuracy and precision of information obtained by quantitative histochemical means has been greatly enhanced by a thorough knowledge of the mechanisms of histochemical reactions obtained by fundamental work on matrix chemistry, and well-considered application of optical measuring tools and conditions of measurement. These are the principles put forward by van Duijn since the pioneer period of histochemistry and to whom this review is dedicated.
通过定量组织化学和细胞化学进行的研究极大地促进了对肝实质细胞代谢变化的认识。在本文中，将对这方面的最新研究进行综述，重点关注三个主题：多倍体作为代谢异质性的来源、蛋白水解在肝细胞质量调节中的作用以及肝细胞的缺血性损伤。在所有这三个领域中，通过对基质化学基础研究获得的组织化学反应机制的深入了解，以及对光学测量工具和测量条件的精心应用，通过定量组织化学方法获得的信息的准确性和精确性得到了极大提高。这些是范·杜因自组织化学开创时期以来提出的原则，本文献献给他。
