Department of Genetics and Development, University of Illinois, 61801, Urbana, IL, USA.
Plant Cell Rep. 1987 Dec;6(6):462-5. doi: 10.1007/BF00272783.
Protoplasts of Phaseolus vulgaris L. (Top Crop), infected with bean golden mosaic virus, were isolated and fixed by various methods for in situ hybridization. An iodine-125 labeled probe was made from the replicative form of the virus. The localization and quantitation was done by autoradiography. Cell wall removal lowered the background and allowed a more accurate analysis. RNase was used to eliminate the possibility of hybrids to RNA. The evidence suggests a sequence of virus movements starting from rough endoplasm reticulum, moving to the nuclear membrane, and finally with the highest concentration inside the nucleus.
菜豆（顶级作物）原生质体感染菜豆花叶病毒后，采用不同方法固定并原位杂交。用病毒复制型制备放射性碘 125 标记探针。放射自显影进行定位和定量分析。去除细胞壁降低了背景，使分析更准确。用 RNase 消除了杂交到 RNA 的可能性。证据表明病毒的运动顺序是从粗糙内质网开始，移动到核膜，最终在核内浓度最高。
