Department of Developmental Biology, Research School of Biological Sciences, Australian National University, P.O. Box 475, 2601, Canberra City, ACT, Australia.
Planta. 1985 Jan;163(1):1-8. doi: 10.1007/BF00395890.
The mechanism by which sub-cortical actin bundles and membranous organelles are immobilised in the cortical cytoplasm of the alga Chara was studied by perfusing cells with a solution containing 1% Triton X-100. Light and scanning electron microscopy and the release of starch grains and chlorophyll-protein complexes indicated that the detergent extensively solubilised the chloroplasts. However, the sub-cortical actin bundles remained in situ even though they were originally separated from the plasma membrane by the chloroplasts. A fibrous layer between chloroplasts and plasma membrane became readily visible after detergent extraction of the cells and could be released by low-ionic-strength ethylenediaminetetraacetic acid, thioglycollate and trypsin. The same treatments applied to cells not subject to detergent extraction released the membrane-bound organelles and actin bundles and no fibrous meshwork was visible on subsequent extraction with Triton. It is, therefore, concluded that a detergent-insoluble cortical cytoskeleton exists and contributes to the immobility of the actin and cortical organelles in the cells.
用含有 1%Triton X-100 的溶液灌流细胞，研究了皮层细胞质中皮层下肌动蛋白束和膜状细胞器固定的机制。光镜和扫描电镜以及淀粉粒和叶绿素-蛋白复合物的释放表明，去污剂可使叶绿体充分溶解。然而，皮层下肌动蛋白束仍然原位存在，尽管它们最初是由叶绿体与质膜隔开的。用去污剂提取细胞后，叶绿体和质膜之间的纤维层变得清晰可见，并用低离子强度的乙二胺四乙酸、巯基乙醇和胰蛋白酶可以将其释放。将相同的处理方法应用于未用去污剂提取的细胞，可释放膜结合的细胞器和肌动蛋白束，随后用 Triton 进行提取时，看不到纤维网格。因此，可以得出结论，存在一种去污剂不溶性皮质细胞骨架，它有助于肌动蛋白和皮质细胞器在细胞中的固定。
