Department of Biological Sciences, University of Stirling, FK9 4LA, Stirling, UK.
Planta. 1985 Feb;163(2):214-7. doi: 10.1007/BF00393509.
Dark respiration rates of guard-cell protoplasts of Commelina communis L. were measured over a temperature range (15-30° C) using a Cartesian-diver microrespirometry technique. Measurements were made using a few microliters of suspension medium containing between 400 and 3 700 protoplasts. Respiration rates were approximately linear for at least 1 h at all temperatures. Respiration rates increased rapidly between 20 and 25° C to relatively high levels (6.11·10(-6) μmol O2 h(-1) protoplast(-1)=1259 μmol O2 mg(-1) chlorophyll h(-1)=22.97 μmol O2 mg(-1) protein h(-1)) with no further increases above this temperature. Respiration rates were much lower in protoplasts 15-16 h old than in freshly prepared ones indicating considerable deterioration of their viability over this time period.
使用一种直角坐标式微室呼吸测量技术，在（15-30℃）温度范围内，对鸭跖草属保卫细胞原生质体的暗呼吸速率进行了测量。测量时使用了几微升含 400 至 3700 个原生质体的悬浮介质。在所有温度下，至少 1 小时内，呼吸速率呈近似线性。在 20-25℃之间，呼吸速率迅速增加，达到相对较高的水平（6.11·10(-6) μmol O2 h(-1) protoplast(-1)=1259 μmol O2 mg(-1) chlorophyll h(-1)=22.97 μmol O2 mg(-1) protein h(-1)），此后温度再升高，呼吸速率不再增加。15-16 小时龄的原生质体的呼吸速率远低于刚制备的原生质体，表明在此时间段内，其活力有相当大的下降。
