Warfel A H, Zucker-Franklin D
J Immunol. 1986 Jul 15;137(2):651-5.
Lipopolysaccharide (LPS) treatment of resident mouse peritoneal macrophages (M phi) was found to suppress intracellular as well as secreted lysozyme (LZM). Interferon (IFN) had a similar effect. LZM was identified by the capacity of cell lysates or medium to lyse Micrococcus lysodeikticus, and by the presence of a 14.5 Kd protein band which co-migrated with human LZM in SDS-PAGE and which reacted positively in Western blots with antiserum to human LZM. The size of the 14.5 Kd band decreased sequentially with increasing concentrations of LPS to which the cells were exposed. Although the LPS influence on LZM levels was dose-dependent, the intracellular LZM pool responded more readily than secreted LZM. Maximal intracellular LZM suppression of 80% was obtained with 10 micrograms LPS, whereas secreted LZM was reduced by only 66%. An IFN concentration of 100 U reduced secreted LZM by 24%, whereas 10,000 U of IFN decreased the amount of LZM secreted by 71%. Thioglycolate-elicited M phi had 75% less intracellular LZM than untreated resident M phi. Moreover, thioglycolate-elicited M phi were hyporesponsive to the suppressive effects of LPS added in vitro. Because both LPS and IFN have been shown to stimulate numerous M phi functions, the data are of interest because they support the concept, based on other studies, that agents which are capable of enhancing some M phi activities may concomitantly down-regulate other functions.
研究发现，用脂多糖（LPS）处理小鼠腹腔常驻巨噬细胞（M phi）可抑制细胞内以及分泌型溶菌酶（LZM）。干扰素（IFN）也有类似作用。通过细胞裂解物或培养基裂解溶壁微球菌的能力，以及在十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - PAGE）中与人类LZM共迁移且在蛋白质免疫印迹中与人LZM抗血清呈阳性反应的14.5 Kd蛋白条带的存在来鉴定LZM。随着细胞暴露于LPS浓度的增加，14.5 Kd条带的大小依次减小。尽管LPS对LZM水平的影响呈剂量依赖性，但细胞内LZM库比分泌型LZM反应更迅速。用10微克LPS可使细胞内LZM最大抑制率达到80%，而分泌型LZM仅减少66%。100单位的IFN可使分泌型LZM减少24%，而10,000单位的IFN可使分泌的LZM量减少71%。巯基乙酸盐诱导的M phi细胞内LZM比未处理的常驻M phi少75%。此外，巯基乙酸盐诱导的M phi对体外添加的LPS的抑制作用反应低下。由于LPS和IFN均已显示可刺激多种M phi功能，这些数据很有意义，因为它们基于其他研究支持了这样一种概念，即能够增强某些M phi活性的试剂可能会同时下调其他功能。
