通过测量与细胞数量相关的生长培养基颜色变化来测定干扰素的抗增殖活性。

The antiproliferative activity of interferons assayed by measuring growth medium color changes related to cell number.

作者信息

Dawson K M, Bawden L J, Featherstone D A

出版信息

J Interferon Res. 1986 Apr;6(2):137-42. doi: 10.1089/jir.1986.6.137.

DOI:10.1089/jir.1986.6.137

Abstract

A simple colorimetric assay for estimating cell numbers has been developed based on the observation that the indicator color in cell culture medium is proportional to viable cell number. The assay is performed in multiwell plates to take advantage of the rapid color measurement and computerized data handling capabilities of multiwell scanning spectrophotometers. Since no centrifugation or washing steps are involved, the technique is particularly useful for cells that grow in suspension, although it is equally applicable to monolayer cultures. The assay was developed to measure the antiproliferative activity of interferons on Daudi lymphoblastoid cells but could equally well be applied to other cell growth inhibition or stimulation assays.

摘要

基于细胞培养基中的指示颜色与活细胞数量成正比这一观察结果，已开发出一种用于估算细胞数量的简单比色测定法。该测定法在多孔板中进行，以利用多孔扫描分光光度计快速颜色测量和计算机化数据处理能力。由于不涉及离心或洗涤步骤，该技术对悬浮生长的细胞特别有用，尽管它同样适用于单层培养。该测定法最初是为测量干扰素对Daudi淋巴母细胞样细胞的抗增殖活性而开发的，但同样可用于其他细胞生长抑制或刺激测定。