白蛋白与分离的大鼠肝细胞相互作用的表征，以揭示白蛋白介导的肝脏转运机制。

Characterization of the interaction of albumin with isolated rat liver cells to reveal the mechanism of albumin-mediated hepatic transport.

作者信息

Mizuma T, Horie T, Hayashi M, Awazu S

出版信息

J Pharmacobiodyn. 1986 Mar;9(3):244-8. doi: 10.1248/bpb1978.9.244.

DOI:10.1248/bpb1978.9.244

Abstract

The binding of bovine serum albumin (BSA), containing 125I-BSA, to isolated rat hepatocytes was studied over a 300-fold concentration range of BSA to characterize the interaction between albumin and the liver cells in albumin-mediated hepatic transport. The binding of BSA with a high affinity to the cell surface of hepatocytes was not found in the binding behavior. The bound fraction of BSA with hepatocytes was about 1% over those concentration range of BSA.

摘要

研究了含有¹²⁵I-牛血清白蛋白（BSA）的牛血清白蛋白与分离的大鼠肝细胞的结合情况，该研究涵盖了300倍浓度范围的BSA，以表征白蛋白与白蛋白介导的肝脏转运中肝细胞之间的相互作用。在结合行为中未发现BSA与肝细胞表面具有高亲和力的结合。在那些BSA浓度范围内，BSA与肝细胞的结合分数约为1%。