Crop Breeding Research Division, Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization (NARO), Hitsujigaoka 1, Toyohira-ku, Sapporo 062-8555, Japan ; School of Applied Biosciences, Kyungpook National University, Daegu 702-701, Republic of Korea.
FEBS Open Bio. 2013 Oct 9;3:438-42. doi: 10.1016/j.fob.2013.10.003. eCollection 2013.
Cold shock proteins (CSPs) of bacteria are produced in response to cold and function as RNA chaperones that are essential for cold adaptation. Arabidopsis thaliana COLD SHOCK DOMAIN PROTEIN 3 (AtCSP3) shares a domain with bacterial CSPs and is involved in acquisition of freezing tolerance. Our previous study revealed that many of the genes that are down regulated in an AtCSP3 knockout mutant (atcsp3-2) are functionally associated with responses to salt and drought as well as cold. Here, we examined the involvement of AtCSP3 in salt and drought stress tolerance. We found that AtCSP3 is induced during salt and drought stresses, and is regulated by ABA. A knockout mutant of AtCSP3 (atcsp3-2) showed lower survival rates after salt and drought stress treatments. Conversely, the AtCSP3-overexpressing plants displayed higher survival rates after treatment with these stresses. Most of the genes that were down regulated in the atcsp3-2 mutant were found to be inducible upon salt and drought stresses, and upregulated in the AtCSP3-overexpressors. Together, our data demonstrates that AtCSP3 is involved in the regulation of salt and drought stress tolerance in Arabidopsis.
细菌的冷休克蛋白（CSPs）是在应对寒冷时产生的，作为 RNA 伴侣蛋白发挥作用，对于冷适应至关重要。拟南芥的冷休克结构域蛋白 3（AtCSP3）与细菌的 CSP 具有相同的结构域，并且参与获得抗冻性。我们之前的研究表明，在 AtCSP3 敲除突变体（atcsp3-2）中下调的许多基因在功能上与对盐、干旱和寒冷的反应有关。在这里，我们研究了 AtCSP3 参与盐和干旱胁迫耐受性的情况。我们发现 AtCSP3 在盐和干旱胁迫下被诱导，并受到 ABA 的调节。AtCSP3 敲除突变体（atcsp3-2）在盐和干旱胁迫处理后存活率较低。相反，AtCSP3 过表达植株在这些胁迫处理后表现出更高的存活率。在 atcsp3-2 突变体中下调的大多数基因在盐和干旱胁迫下是可诱导的，并在 AtCSP3 过表达植株中上调。总之，我们的数据表明 AtCSP3 参与了拟南芥对盐和干旱胁迫耐受性的调节。
