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番茄( L.)基因家族的特征及其对激素和非生物胁迫响应的 s 的表达分析。

Characterization of the Gene Family in Tomato ( L.) and the Expression Analysis of s in Response to Hormone and Abiotic Stresses.

机构信息

College of Horticulture, Gansu Agricultural University, 1 Yinmen Village, Anning District, Lanzhou 730070, China.

College of Agriculture, Guangxi University, Nanning 530004, China.

出版信息

Int J Mol Sci. 2023 Nov 7;24(22):16063. doi: 10.3390/ijms242216063.

Abstract

Fasciclin-like arabinogalactan proteins (FLAs), a subclass of arabinogalactan proteins (AGPs), participate in mediating plant growth, development, and response to abiotic stress. However, the characterization and function of in tomato are currently unknown. In this study, members of the tomato family are characterized and analyzed in relation to their response to phytohormonal and abiotic stresses. The results show that a total of 24 members were characterized in tomato. The structural domain analysis showed that these members have a high protein similarity. The expression profiles of different tissues indicated that the genes of most members of the tomato gene family are highly expressed in roots, but to a lower extent in fruits. qRT-PCR analysis revealed that all 24 tomato genes are responsive to ABA and MeJA. showed a positive response to salt and cold stress. , , and are significantly induced under darkness. and are significantly induced under drought stress. This study provides a basis for a further understanding of the role of tomato homologous genes in plant response to abiotic stress and lays the foundation for further research on the function of in tomato.

摘要

纤维连接蛋白样阿拉伯半乳聚糖蛋白(FLAs)是阿拉伯半乳聚糖蛋白(AGPs)的一个亚类,参与介导植物的生长、发育和对非生物胁迫的响应。然而,番茄中 的特征和功能目前尚不清楚。在本研究中,对番茄 的成员进行了特征描述和分析,以研究它们对植物激素和非生物胁迫的响应。结果表明,在番茄中鉴定出了 24 个 成员。结构域分析表明,这些成员具有高度的蛋白质相似性。不同组织的表达谱分析表明,番茄 基因家族的大多数成员的基因在根中高度表达,但在果实中表达程度较低。qRT-PCR 分析表明,24 个番茄 基因均对 ABA 和 MeJA 有响应。 对盐和冷胁迫表现出正响应。 、 和 在黑暗下显著诱导。 和 在干旱胁迫下显著诱导。本研究为进一步了解番茄 同源基因在植物应对非生物胁迫中的作用提供了基础,并为进一步研究 基因在番茄中的功能奠定了基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bd9/10671457/0aa649f507f6/ijms-24-16063-g001.jpg

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