Department of Polymer Science and Engineering, Kyunpook National University , #1370 Sangyuk-dong, Buk-gu, Daegu 702-701, Korea.
ACS Appl Mater Interfaces. 2013 Dec 26;5(24):13135-9. doi: 10.1021/am404174n. Epub 2013 Nov 22.
Micrometer-sized, 4-cyno-4-pentylbiphenyl (5CB) droplets were developed for glucose detection in an aqueous medium by coating with poly(acrylicacid-b-4-cynobiphenyl-4-oxyundecylacrylate) (PAA-b-LCP) at the 5CB/water interface and covalently immobilizing glucose oxidase (GOx) to the PAA chains. This functionalized liquid-crystal (LC) droplet detected glucose from a radial to bipolar configurational change by polarized optical microscopy under crossed polarizers at concentrations as low as 0.03 mM and response times of ~3 min and showed the selective detection of glucose against galactose. This new and sensitive LC-droplet-based glucose biosensor has the merits of low production cost and easy detection by the naked eye and might be useful for prescreening the glucose level in the human body.
微米级的 4-氰基-4-戊基联苯（5CB）液滴被开发用于在水介质中检测葡萄糖，方法是在 5CB/水界面处用聚（丙烯酸-b-4-氰基联苯-4-氧基十一烷基丙烯酰胺）（PAA-b-LCP）进行涂层，并将葡萄糖氧化酶（GOx）共价固定到 PAA 链上。这种功能化的液晶（LC）液滴通过在正交偏光显微镜下从径向到双极构象变化来检测葡萄糖，在低至 0.03 mM 的浓度下和~3 分钟的响应时间下显示出对葡萄糖的选择性检测，而对半乳糖则没有响应。这种新型的基于 LC 液滴的葡萄糖生物传感器具有生产成本低和肉眼易于检测的优点，可能有助于对人体中的葡萄糖水平进行预筛选。
