用于宽视场显微镜荧光成像的可调谐、低成本、多通道、宽带液晶快门

Tunable, Low-Cost, Multi-Channel, Broadband Liquid Crystal Shutter for Fluorescence Imaging in Widefield Microscopy.

作者信息

Gong Yan, Li Bo, Yao Cheng-You, Yang Weiyang, Fan Qi Hua, Qiu Zhen, Li Wen

机构信息

Electrical and Computer Engineering Department, Michigan State University, 428 S Shaw Ln, #2120, East Lansing, MI 48824, USA.

Biomedical Engineering Department, Michigan State University, 775 Woodlot Dr, East Lansing, MI 48824, USA.

出版信息

Micromachines (Basel). 2022 Aug 13;13(8):1310. doi: 10.3390/mi13081310.

DOI:10.3390/mi13081310

PMID:36014232

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9415866/

Abstract

Bistable liquid crystal (LC) shutters have attracted much interest due to their low energy consumption and fast response time. In this paper, we demonstrate an electrically tunable/switchable biostable LC light shutter in biological optics through a three-step easy-assembly, inexpensive, multi-channel shutter. The liquid crystal exhibits tunable transparency (100% to 10% compared to the initial light intensity) under different voltages (0 V to 90 V), indicating its tunable potential. By using biomedical images, the response time, resolution, and light intensity changes of the LC under different voltages in three common fluorescence wavelengths are displayed intuitively. Particularly, the shutter's performance in tumor images under the near-infrared band shows its application potential in biomedical imaging fields.

摘要

双稳态液晶（LC）快门因其低能耗和快速响应时间而备受关注。在本文中，我们通过一种三步简易组装、成本低廉的多通道快门，展示了一种在生物光学中电可调/可切换的双稳态LC光快门。该液晶在不同电压（0 V至90 V）下呈现出可调透明度（与初始光强相比为100%至10%），表明其具有可调潜力。通过使用生物医学图像，直观地展示了液晶在三种常见荧光波长下不同电压下的响应时间、分辨率和光强变化。特别是，该快门在近红外波段下肿瘤图像中的性能展示了其在生物医学成像领域的应用潜力。