Bernardo K L, Ma P M, Woolsey T A
J Neurosci Methods. 1986 Apr;16(2):89-101. doi: 10.1016/0165-0270(86)90042-7.
Horseradish peroxidase crystals or HRP-NP40 detergent chips were directly applied to brain slices from mice to label primary afferent fibers and their terminal arbors in the brainstem trigeminal complex, and neurons of the ventrobasal complex of the thalamus, their axons in the internal capsule, and their terminals in the primary somatosensory cortex. Anterograde and retrograde labeling of fibers, as well as retrograde labeling of somata, were observed. In vitro labeling of selected non-trigeminal structures and fiber pathways was also demonstrated. Experimental variables have been dealt with in some detail, as have specific advantages and disadvantages of the technique. This in vitro HRP labeling method for neuronal fiber systems is a useful adjunct to currently employed in vivo labeling techniques in the mammalian central nervous system.
将辣根过氧化物酶晶体或辣根过氧化物酶 - NP40去污剂片直接应用于小鼠脑片，以标记脑干三叉神经复合体中的初级传入纤维及其终末分支、丘脑腹侧基底复合体的神经元、它们在内囊中的轴突以及它们在初级体感皮层中的终末。观察到纤维的顺行和逆行标记以及胞体的逆行标记。还展示了对选定的非三叉神经结构和纤维通路的体外标记。已详细讨论了实验变量以及该技术的具体优缺点。这种用于神经元纤维系统的体外辣根过氧化物酶标记方法是目前哺乳动物中枢神经系统体内标记技术的有用辅助手段。
